| Antigen detectable with this ELISA kit: |
B-Raf Proto Oncogene Serine/Threonine Protein Kinase (BRAF) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 0, 1, 10ng/mL, 2, 5ng/mL, 25ng/mL, 313-20ng/mL The standard curve concentrations used for the ELISA&rsquo, 313ng/mL, 5ng/mL, 625ng/mL, s were 20ng/mL |
| Sensitivity: |
124ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
Tissue homogenates and other biological fluids |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to B-Raf Proto Oncogene Serine/Threonine Protein Kinase (BRAF), The concentration of B-Raf Proto Oncogene Serine/Threonine Protein Kinase (BRAF) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to B-Raf Proto Oncogene Serine/Threonine Protein Kinase (BRAF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain B-Raf Proto Oncogene Serine/Threonine Protein Kinase (BRAF), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
B-Raf Proto Oncogene Serine/Threonine Protein Kinase (BRAF) |
| Short name: |
B-Raf Proto Oncogene Serine/Threonine Protein Kinase (BRAF) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
B-Raf Proto Oncogene Serine/Threonine Protein phosphorylation catalyst (v-raf murine sarcoma viral oncogene homolog B1) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
B-RAF1 and BRAF1 and NS7 and RAFB1, BRAF and IDBG-44351 and ENSG00000157764 and 673, BRAF and IDBG-636391 and ENSBTAG00000021761 and, Braf and IDBG-138657 and ENSMUSG00000002413 and 109880, alpha-beta T cell differentiation and biological process this GO :0043368 and positive T cell selection and biological process this GO :0043434 and response to peptide hormone and biological process this GO :0043524 and negative regulation of neuron apoptotic process and biological process this GO :0044297 and cell body and cellular component this GO :0046632 and alpha-beta T cell differentiation and biological process this GO :0046982 and protein heterodimerization activity and molecular function this GO :0048011 and neurotrophin TRK receptor signaling pathway and biological process this GO :0051291 and protein heterooli this GO merization and biological process this GO :0051496 and positive regulation of stress fiber assembly and biological process this GO :0051591 and response to cAMP and biological process this GO :0060291 and long-term synaptic potentiation and biological process this GO :0070374 and positive regulation of ERK1 and ERK2 cascade and biological process this GO :0070849 and response to epidermal growth factor and biological process this GO :0071277 and cellular response to calcium ion and biological process this GO :1900026 and positive regulation of substrate adhesion-dependent cell spreading and biological process this GO :2000301 and negative regulation of synaptic vesicle exocytosis and biological process this GO :2000352 and negative regulation of endothelial cell apoptotic process and biological process, nuclei, this GO :0000165 and MAPK cascade and biological process this GO :0000186 and activation of MAPKK activity and biological process this GO :0002318 and myeloid progenitor cell differentiation and biological process this GO :0004672 and protein kinase activity and molecular function this GO :0004674 and protein serine/threonine kinase activity and molecular function this GO :0004709 and MAP kinase kinase kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0005057 and receptor signaling protein activity and molecular function this GO :0005509 and calcium ion binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005739 and mitochondrion and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006468 and protein phosphorylation and biological process this GO :0007165 and signal transduction and biological process this GO :0007264 and small GTPase mediated signal transduction and biological process this GO :0007268 and synaptic transmission and biological process this GO :0008542 and visual learning and biological process this GO :0008543 and fibroblast growth factor receptor signaling pathway and biological process this GO :0009887 and organ morphogenesis and biological process this GO :0010628 and positive regulation of gene expression and biological process this GO :0010764 and negative regulation of fibroblast migration and biological process this GO :0016020 and membrane and cellular component this GO :0016772 and transferase activity, this GO :0004672 : protein kinase activity, this GO :0004672 : protein kinase activity and also this GO :0004674 : protein serine/threonine kinase activity and also this GO :0004709 : MAP kinase kinase kinase activity and also this GO :0004713 : protein tyrosine kinase activity and also this GO :0005057 : receptor signaling protein activity and also this GO :0005509 : calcium ion binding and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0016772 : transferase activity, this GO :0004674 : protein serine/threonine kinase activity, this GO :0004709 : MAP kinase kinase kinase activity, this GO :0004713 : protein tyrosine kinase activity, this GO :0005057 : receptor signaling protein activity, this GO :0005509 : calcium ion binding, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0016772 : transferase activity, this GO :0031434 : mitogen-activated protein kinase kinase binding, this GO :0042802 : identical protein binding, this GO :0046982 : protein heterodimerization activity, transferase activity, transferring phosphorus-containing groups, transferring phosphorus-containing groups and also this GO :0031434 : mitogen-activated protein kinase kinase binding and also this GO :0042802 : identical protein binding and also this GO :0046982 : protein heterodimerization activity, transferring phosphorus-containing groups and molecular function this GO :0030154 and cell differentiation and biological process this GO :0031434 and mitogen-activated protein kinase kinase binding and molecular function this GO :0033138 and positive regulation of peptidyl-serine phosphorylation and biological process this GO :0035019 and somatic stem cell maintenance and biological process this GO :0035556 and intracellular signal transduction and biological process this GO :0035690 and cellular response to drug and biological process this GO :0042127 and regulation of cell proliferation and biological process this GO :0042802 and identical protein binding and molecular function this GO :0043005 and neuron projection and cellular component this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0043367 and CD4-positive, v-raf murine sarcoma viral oncogene homolog B1 |
| Identity: |
1097 |
| Gene: |
BRAF |
More about : BRAF |
| Long gene name: |
B-Raf proto-oncogene, serine/threonine kinase |
| Synonyms gene name: |
v-raf murine sarcoma viral oncogene homolog B |
| Synonyms: |
BRAF1 |
| Locus: |
7q34 |
| Discovery year: |
1991-07-16 |
| GenBank acession: |
M95712 |
| Entrez gene record: |
673 |
| Pubmed identfication: |
2284096 1565476 |
| RefSeq identity: |
NM_004333 |
| Classification: |
RAF family Mitogen-activated protein kinase kinase kinases |
| Havana BLAST/BLAT: |
OTTHUMG00000157457 |
| Locus Specific Databases: |
LRG_299 |