| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
LEO1 |
| Specificity: |
This antibody reacts specifically with LEO1 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human LEO1 |
| Gene ID: |
123169 |
| Swiss Prot: |
Q8WVC0 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Connects PAF1C to Wnt signaling, In case of infection by influenza A strain H3N2, Involved in polyadenylation of mRNA precursors, PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, PAF1C associates with viral NS1 protein, PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1, PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3), PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors, PAF1C is required for transcription of Hox and Wnt target genes, PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1), UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1, it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL, thereby regulating gene transcription, Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
LEO1 Polyclonal Antibody |
| Other name: |
Anti-LEO1 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
LEO1 |
| Short name: |
Anti-LEO1 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-LEO1 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
30401 |
| Gene: |
LEO1 |
More about : LEO1 |
| Long gene name: |
LEO1 homolog, Paf1/RNA polymerase II complex component |
| Synonyms gene name: |
Leo1, Paf1/RNA polymerase II complex component, cerevisiae) , homolog (S |
| Locus: |
15q21, 2 |
| Discovery year: |
2005-07-20 |
| GenBank acession: |
AY302186 |
| Entrez gene record: |
123169 |
| Pubmed identfication: |
15632063 24038468 |
| RefSeq identity: |
NM_138792 |
| Classification: |
Paf1/RNA polymerase II complex |
| Havana BLAST/BLAT: |
OTTHUMG00000131843 |