| Long name: |
Biotin Conjugated, LEO1 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
LEO1 |
| Specificity: |
This is a highly specific antibody against LEO1 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
500-550/666 |
| Subcellular location: |
Nucleus |
| Source: |
KLH conjugated synthetic peptide derived from human LEO1 |
| Gene ID number: |
123169 |
| Swiss Prot: |
Q8WVC0 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Connects PAF1C to Wnt signaling, In case of infection by influenza A strain H3N2, Involved in polyadenylation of mRNA precursors, PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, PAF1C associates with viral NS1 protein, PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1, PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3), PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors, PAF1C is required for transcription of Hox and Wnt target genes, PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1), UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1, it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL, thereby regulating gene transcription, Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
LEO1 Conjugated |
| Short name: |
Conjugated, LEO1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, LEO1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
30401 |
| Gene: |
LEO1 |
More about : LEO1 |
| Long gene name: |
LEO1 homolog, Paf1/RNA polymerase II complex component |
| Synonyms gene name: |
Leo1, Paf1/RNA polymerase II complex component, cerevisiae) , homolog (S |
| Locus: |
15q21, 2 |
| Discovery year: |
2005-07-20 |
| GenBank acession: |
AY302186 |
| Entrez gene record: |
123169 |
| Pubmed identfication: |
15632063 24038468 |
| RefSeq identity: |
NM_138792 |
| Classification: |
Paf1/RNA polymerase II complex |
| Havana BLAST/BLAT: |
OTTHUMG00000131843 |