| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
DDX58 |
| Specificity: |
This antibody reacts specifically with DDX58 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human DDX58 |
| Gene ID: |
23586 |
| Swiss Prot: |
O95786 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
A 3'overhang at the 5'triphosphate end decreases and any 5'overhang at the 5' triphosphate end abolishes its activity, Also involved in antiviral signaling in response to viruses containing a dsDNA genome such as Epstein-Barr virus (EBV), Detects both positive and negative strand RNA viruses including members of the families Paramyxoviridae: Human respiratory syncytial virus and measles virus (MeV), Detects dsRNA produced from non-self dsDNA by RNA polymerase III, Flaviviridae: Japanese encephalitis virus (JEV), IFN-alpha and IFN-beta, In addition to the 5'-triphosphate moiety, It also detects rotavirus and reovirus, Its ligands include: 5'-triphosphorylated ssRNA and dsRNA and short dsRNA (<, May play important roles in granulocyte production and differentiation, Orthomyxoviridae: influenza A and B virus, Overhangs at the non-triphosphorylated end of the dsRNA RNA have no major impact on its activity, Rhabdoviridae: vesicular stomatitis virus (VSV), Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1) which activates the IKK-related kinases: TBK1 and IKBKE which phosphorylate interferon regulatory factors: IRF3 and IRF7 which in turn activate transcription of antiviral immunological genes, bacterial phagocytosis and in the regulation of cell migration, blunt-end base pairing at the 5'-end of the RNA is very essential, dengue virus (DENV) and west Nile virus (WNV), hepatitis C virus (HCV), including interferons (IFNs), such as Epstein-Barr virus-encoded RNAs (EBERs), 1 kb in length), Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and proinflammatory cytokines |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
RIG-I FLJ13599 DKFZp434J1111 |
| Also known as: |
DDX58 Polyclonal Antibody |
| Other name: |
Anti-DDX58 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
DDX58 |
| Short name: |
Anti-DDX58 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-DEAD (L-aspartic acid-L-gutamic acid-alanine-L-aspartic acid) box polypeptide 58 (polyclonal) |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
Cell surfaces, DDX58 and IDBG-55854 and ENSG00000107201 and 23586, DDX58 and IDBG-629773 and ENSBTAG00000003366 and 504760, Ddx58 and IDBG-135957 and ENSMUSG00000040296 and 230073, RIG-I and RIGI and RLR-1, acting on acid anhydrides, acting on acid anhydrides and also this GO :0042802 : identical protein binding, acting on acid anhydrides and molecular function this GO :0030334 and regulation of cell migration and biological process this GO :0032480 and negative regulation of type I interferon production and biological process this GO :0032587 and ruffle membrane and cellular component this GO :0032727 and positive regulation of interferon-alpha production and biological process this GO :0032728 and positive regulation of interferon-beta production and biological process this GO :0034344 and regulation of type III interferon production and biological process this GO :0039528 and cytoplasmic pattern recognition receptor signaling pathway in response to virus and biological process this GO :0039529 and RIG-I signaling pathway and biological process this GO :0042802 and identical protein binding and molecular function this GO :0042993 and positive regulation of transcription factor import into nucleus and biological process this GO :0043330 and response to exogenous dsRNA and biological process this GO :0045087 and innate immune response and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0051091 and positive regulation of sequence-specific DNA binding transcription factor activity and biological process, hydrolase activity, this GO :0002230 and positive regulation of defense response to virus by host and biological process this GO :0003676 and nucleic acid binding and molecular function this GO :0003677 and DNA binding and molecular function this GO :0003690 and double-stranded DNA binding and molecular function this GO :0003725 and double-stranded RNA binding and molecular function this GO :0003727 and single-stranded RNA binding and molecular function this GO :0004386 and helicase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0005923 and tight junction and cellular component this GO :0008152 and metabolic process and biological process this GO :0008270 and zinc ion binding and molecular function this GO :0009597 and detection of virus and biological process this GO :0009615 and response to virus and biological process this GO :0015629 and actin cytoskeleton and cellular component this GO :0016032 and viral process and biological process this GO :0016787 and hydrolase activity and molecular function this GO :0016817 and hydrolase activity, this GO :0003676 : nucleic acid binding, this GO :0003676 : nucleic acid binding and also this GO :0003677 : DNA binding and also this GO :0003690 : double-stranded DNA binding and also this GO :0003725 : double-stranded RNA binding and also this GO :0003727 : single-stranded RNA binding and also this GO :0004386 : helicase activity and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0008270 : zinc ion binding and also this GO :0016787 : hydrolase activity and also this GO :0016817 : hydrolase activity, this GO :0003677 : DNA binding, this GO :0003690 : double-stranded DNA binding, this GO :0003725 : double-stranded RNA binding, this GO :0003727 : single-stranded RNA binding, this GO :0004386 : helicase activity, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0008270 : zinc ion binding, this GO :0016787 : hydrolase activity, this GO :0016817 : hydrolase activity, this GO :0042802 : identical protein binding, DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 |
| Identity: |
19102 |
| Gene: |
DDX58 |
More about : DDX58 |
| Long gene name: |
DExD/H-box helicase 58 |
| Synonyms gene name: |
DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 |
| Synonyms name: |
RNA helicase RIG-I retinoic acid inducible gene I |
| Locus: |
9p21, 1 |
| Discovery year: |
2004-05-10 |
| GenBank acession: |
AF038963 |
| Entrez gene record: |
23586 |
| Pubmed identfication: |
21690088 |
| RefSeq identity: |
NM_014314 |
| Classification: |
DEAD-box helicases |
| Havana BLAST/BLAT: |
OTTHUMG00000019746 |