| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Mast Cell Tryptase |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA (protein), IHC-P, WB |
| Recommended dilutions: |
request BSA-free format for coating, 1-0, 5-1ug/ml, 5ug/ml (human protein tested), ELISA: 0, IHC (FFPE): 1-2ug/ml, Western blot: 0 |
| Added buffer: |
025% sodium azide, 5% BSA and 0, Lyophilized from 1X Phosphate Buffered Saline (PBS) with 2 |
| Intented use: |
This Mast Cell Tryptase antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q15661 |
| Purity: |
Antigen affinity |
| Description: |
Beta tryptases appear to be the main isoenzymes expressed in mast cells, In addition, Several tryptase genes are clustered on chromosome 16p13, The alleles of this gene exhibit an unusual amount of sequence variation, These genes are characterized by several distinct features, They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability, This feature is characteristic of tryptases but is unusual in other genes, Tryptases are enzymatically active only as heparin-stabilized tetramers, Tryptases comprise a family of trypsin-like serine proteases, Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders, alpha and beta 1, alpha tryptases predominate, and they are resistant to all known endogenous proteinase inhibitors, such that the alleles were once thought to represent two separate genes, the peptidase family S1, these genes have an intron immediately upstream of the initiator Met codon, whereas in basophils, which separates the site of transcription initiation from protein coding sequence, 3, Tryptase alpha-1 and tryptase beta-1 are enzymes that in humans are encoded by the same TPSAB1 gene |
| Immunogen: |
Amino acids H65-P275 from the human protein were used as the immunogen for the Mast Cell Tryptase antibody |
| Storage: |
After reconstitution, Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, store at 4oC, the Mast Cell Tryptase antibody may be kept for up to one month refrigerated at +4 degrees C, For long-term, Prior to reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Mast Tryptase |
| Short name: |
Mast Cell Tryptase Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
Mast cellular Tryptase (Antibody to) |
| Alternative technique: |
antibodies |