| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
MSH2 |
| Specificity: |
This antibody reacts specifically with MSH2 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human MSH-2 |
| Gene ID: |
4436 |
| Swiss Prot: |
P43246 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
ATP binding and hydrolysis play a pivotal role in mismatch repair functions, After mismatch binding, Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair, In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis, MutS alpha may also play a role in DNA homologous recombination repair, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA, MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long, The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->, This transition is crucial for mismatch repair, When bound, and resynthesis, excision, heterodimers bend the DNA helix and shields approximately 20 base pairs, including strand discrimination, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone, which is thought to be responsible for directing the downstream MMR events, ATP exchange, Component of the post-replicative DNA mismatch repair system (MMR) |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
HNPCC HNPCC1 |
| Also known as: |
MSH2 Polyclonal Antibody |
| Other name: |
Anti-MSH2 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
MSH2 |
| Short name: |
Anti-MSH2 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, coli) (polyclonal), colon cancer, nonpolyposis type 1 (E, antibody to-mutS homolog 2 |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
ADP binding, COCA1 and FCC1 and HNPCC and HNPCC1 and LCFS2, MSH2 and IDBG-50900 and ENSG00000095002 and 4436, MSH2 and IDBG-633627 and ENSBTAG00000002742 and 533115, Msh2 and IDBG-201483 and ENSMUSG00000024151 and 17685, coli), colon cancer, nonpolyposis type 1 (E, nuclei, this GO :0000228 and nuclear chromosome and cellular component this GO :0000287 and magnesium ion binding and molecular function this GO :0000400 and four-way junction DNA binding and molecular function this GO :0000403 and Y-form DNA binding and molecular function this GO :0000404 and heteroduplex DNA loop binding and molecular function this GO :0000406 and double-strand/single-strand DNA junction binding and molecular function this GO :0000710 and meiotic mismatch repair and biological process this GO :0001701 and in utero embryonic development and biological process this GO :0002204 and somatic recombination of immunoglobulin genes involved in immune response and biological process this GO :0003677 and DNA binding and molecular function this GO :0003684 and damaged DNA binding and molecular function this GO :0003690 and double-stranded DNA binding and molecular function this GO :0003697 and single-stranded DNA binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0006119 and oxidative phosphorylation and biological process this GO :0006200 and ATP catabolic process and biological process this GO :0006281 and DNA repair and biological process this GO :0006298 and mismatch repair and biological process this GO :0006301 and postreplication repair and biological process this GO :0006302 and double-strand break repair and biological process this GO :0006311 and meiotic gene conversion and biological process this GO :0006974 and cellular response to DNA damage stimulus and biological process this GO :0007050 and cell cycle arrest and biological process this GO :0007281 and germ cell development and biological process this GO :0008022 and protein C-terminus binding and molecular function this GO :0008094 and DNA-dependent ATPase activity and molecular function this GO :0008340 and determination of adult lifespan and biological process this GO :0008584 and male this GO nad development and biological process this GO :0008630 and intrinsic apoptotic signaling pathway in response to DNA damage and biological process this GO :0010165 and response to X-ray and biological process this GO :0010224 and response to UV-B and biological process this GO :0016020 and membrane and cellular component this GO :0016446 and somatic hypermutation of immunoglobulin genes and biological process this GO :0016447 and somatic recombination of immunoglobulin gene segments and biological process this GO :0016887 and ATPase activity and molecular function this GO :0019237 and centromeric DNA binding and molecular function this GO :0019724 and B cell mediated immunity and biological process this GO :0019899 and enzyme binding and molecular function this GO :0019901 and protein kinase binding and molecular function this GO :0030183 and B cell differentiation and biological process this GO :0030983 and mismatched DNA binding and molecular function this GO :0031573 and intra-S DNA damage checkpoint and biological process this GO :0032137 and guanine/thymine mispair binding and molecular function this GO :0032139 and dinucleotide insertion or deletion binding and molecular function this GO :0032142 and single guanine insertion binding and molecular function this GO :0032143 and single thymine insertion binding and molecular function this GO :0032181 and dinucleotide repeat insertion binding and molecular function this GO :0032301 and MutSalpha complex and cellular component this GO :0032302 and MutSbeta complex and cellular component this GO :0032357 and oxidized purine DNA binding and molecular function this GO :0032405 and MutLalpha complex binding and molecular function this GO :0042771 and intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator and biological process this GO :0042803 and protein homodimerization activity and molecular function this GO :0043524 and negative regulation of neuron apoptotic process and biological process this GO :0043531 and ADP binding and molecular function this GO :0043570 and maintenance of DNA repeat elements and biological process this GO :0045128 and negative regulation of reciprocal meiotic recombination and biological process this GO :0045190 and isotype switching and biological process this GO :0045910 and negative regulation of DNA recombination and biological process this GO :0051096 and positive regulation of helicase activity and biological process, this GO :0000287 : magnesium ion binding, this GO :0000287 : magnesium ion binding and also this GO :0000400 : four-way junction DNA binding and also this GO :0000403 : Y-form DNA binding and also this GO :0000404 : heteroduplex DNA loop binding and also this GO :0000406 : double-strand/single-strand DNA junction binding and also this GO :0003677 : DNA binding and also this GO :0003684 : damaged DNA binding and also this GO :0003690 : double-stranded DNA binding and also this GO :0003697 : single-stranded DNA binding and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0008022 : protein C-terminus binding and also this GO :0008094 : DNA-dependent ATPase activity and also this GO :0016887 : ATPase activity and also this GO :0019237 : centromeric DNA binding and also this GO :0019899 : enzyme binding and also this GO :0019901 : protein kinase binding and also this GO :0030983 : mismatched DNA binding and also this GO :0032137 : guanine/thymine mispair binding and also this GO :0032139 : dinucleotide insertion or deletion binding and also this GO :0032142 : single guanine insertion binding and also this GO :0032143 : single thymine insertion binding and also this GO :0032181 : dinucleotide repeat insertion binding and also this GO :0032357 : oxidized purine DNA binding and also this GO :0032405 : MutLalpha complex binding and also this GO :0042803 : protein homodimerization activity and also this GO :0043531 : ADP binding, this GO :0000400 : four-way junction DNA binding, this GO :0000403 : Y-form DNA binding, this GO :0000404 : heteroduplex DNA loop binding, this GO :0000406 : double-strand/single-strand DNA junction binding, this GO :0003677 : DNA binding, this GO :0003684 : damaged DNA binding, this GO :0003690 : double-stranded DNA binding, this GO :0003697 : single-stranded DNA binding, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0008022 : protein C-terminus binding, this GO :0008094 : DNA-dependent ATPase activity, this GO :0016887 : ATPase activity, this GO :0019237 : centromeric DNA binding, this GO :0019899 : enzyme binding, this GO :0019901 : protein kinase binding, this GO :0030983 : mismatched DNA binding, this GO :0032137 : guanine/thymine mispair binding, this GO :0032139 : dinucleotide insertion or deletion binding, this GO :0032142 : single guanine insertion binding, this GO :0032143 : single thymine insertion binding, this GO :0032181 : dinucleotide repeat insertion binding, this GO :0032357 : oxidized purine DNA binding, this GO :0032405 : MutLalpha complex binding, this GO :0042803 : protein homodimerization activity, this GO :0043531 : ADP binding, mutS homolog 2 |
| Identity: |
7325 |
| Gene: |
MSH2 |
More about : MSH2 |
| Long gene name: |
mutS homolog 2 |
| Synonyms gene: |
COCA1 |
| Synonyms gene name: |
coli) , coli) homolog 2 (colon cancer, colon cancer, mutS (E, nonpolyposis type 1 (E, nonpolyposis type 1) mutS homolog 2 |
| Locus: |
2p21-p16, 3 |
| Discovery year: |
1993-07-28 |
| GenBank acession: |
U03911 |
| Entrez gene record: |
4436 |
| Pubmed identfication: |
8484120 9843200 |
| Classification: |
MutS homologs |
| Havana BLAST/BLAT: |
OTTHUMG00000128861 |
| Locus Specific Databases: |
HNPCC Colon cancer gene variant databases MSH2 database at LOVD-China LOVD - Leiden Open Variation Database LRG_218 , Hereditary Non-Polyposis Colorectal Cancer |