| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
HIF-1 Alpha |
| Specificity: |
This antibody reacts specifically with HIF-1 Alpha |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus, Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human of human HIF-1 Alpha |
| Gene ID: |
3091 |
| Swiss Prot: |
Q16665 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Activation requires recruitment of transcriptional coactivators such as CREBPB and EP3, Activity is enhanced by interaction with both, Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters, HILPDA, Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP, Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia, NCOA1 or NCOA2, Plays an essential role in embryonic vascularization, Under hypoxic conditions, activates the transcription of over 4 genes, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia, glucose transporters, glycolytic enzymes, including erythropoietin, tumor angiogenesis and pathophysiology of ischemic disease, vascular endothelial growth factor, Functions as a master transcriptional regulator of the adaptive response to hypoxia |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
HYPB HIF-1 KIAA1732 FLJ23184 KMT3A |
| Also known as: |
HIF-1 Alpha Polyclonal Antibody |
| Other name: |
Anti-HIF-1 Alpha Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Description: |
- or alpha protein sometimes glycoprotein present in blood, The Anti-HIF-1 Alpha (Polyclonal) Fluor 594 is a &alpha |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
HIF-1 Alpha |
| Short name: |
Anti-HIF-1 Alpha (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-HIF-1 a (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
18420 |
| Gene: |
SETD2 |
More about : SETD2 |
| Long gene name: |
SET domain containing 2 |
| Locus: |
3p21, 31 |
| Discovery year: |
2006-02-15 |
| GenBank acession: |
AJ238403 |
| Entrez gene record: |
29072 |
| Pubmed identfication: |
16118227 11461154 |
| RefSeq identity: |
NM_014159 |
| Classification: |
Lysine methyltransferases |
| Havana BLAST/BLAT: |
OTTHUMG00000133514 |
| Locus Specific Databases: |
LRG_775 |