| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Cyclin E |
| Specificity: |
This antibody reacts specifically with Cyclin E |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus |
| Antigen Source: |
KLH conjugated synthetic peptide derived from rat Cyclin E |
| Gene ID: |
25729 |
| Swiss Prot: |
P39949 |
| Applications: |
IF(IHC-P), FCM |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200), FCM(1:20-100) |
| Cross reactive species: |
Rat (Rattus norvegicus), Mouse (Mus musculus) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Cyclins function as regulators of CDK kinases, Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event, Overexpression of this gene has been observed in many tumors, The encoded protein has a 15 aa longer N-terminus, This cyclin forms a complex with and functions as a regulatory subunit of CDK2, This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase, This protein was found to associate with, Transcript Variant: This variant (1) contains a different 5' end region, Two additional splice variants were reported but detailed nucleotide sequence information is not yet available, Two alternatively spliced transcript variants of this gene, and be involved in, and thus may contribute to tumorigenesis, as compared to isoform 2, have been described, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), when compared to variant 2, which encode distinct isoforms, which includes an upstream in-frame translation start codon, which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB, which results in chromosome instability, whose activity is required for cell cycle G1/S transition, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle, The protein encoded by this gene belongs to the highly conserved cyclin family |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
CYCLE, Ccne1, G1/S-specific cyclin-E1, Ccne |
| Also known as: |
Cyclin E Polyclonal Antibody |
| Other name: |
Anti-Cyclin E Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Cyclin E |
| Short name: |
Anti-Cyclin E (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Cyclin E (polyclonal) |
| Alternative technique: |
polyclonals |