| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
DDB1 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
1-0, 5-1ug/ml, 5ug/ml, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Optimal dilution of the DDB1 antibody should be determined by the researcher |
| Intented use: |
This DDB1 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q16531 |
| Purity: |
Antigen affinity |
| Description: |
And this protein complex functions in nucleotide-excision repair and binds to DNA following UV damage, Best vitelliform mascular dystrophy is mapped to the same region as this gene on 11q, Defective activity of this complex causes the repair defect in patients with xeroderma pigmentosum complementation group E (XPE) - an autosomal recessive disorder characterized by photosensitivity and early onset of carcinomas, However, In addition, The protein encoded by this gene also functions as an adaptor molecule for the cullin 4 (CUL4) ubiquitin E3 ligase complex by facilitating the binding of substrates to this complex and the ubiquitination of proteins, but no sequence alternations of this gene are demonstrated in Best disease patients, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the small subunit, The protein encoded by this gene is the large subunit (p127) of the heterodimeric DNA damage-binding (DDB) complex while another protein (p48) forms the small subunit |
| Immunogen: |
Amino acids 1011-1140 of human DDB1 were used as the immunogen for the DDB1 antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the DDB1 antibody may be kept for up to one month refrigerated at +4 degrees C, After reconstitution, For long-term |
| Localization: |
Nuclear and cytoplasmic |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
DDB1 |
| Short name: |
DDB1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
DDB1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
2717 |
| Gene: |
DDB1 |
More about : DDB1 |
| Long gene name: |
damage specific DNA binding protein 1 |
| Synonyms gene name: |
damage-specific DNA binding protein 1 (127kD) |
| Locus: |
11q12, 2 |
| Discovery year: |
1995-07-06 |
| GenBank acession: |
AJ002955 |
| Entrez gene record: |
1642 |
| Pubmed identfication: |
8530102 10574459 |
| RefSeq identity: |
NM_001923 |
| Classification: |
Nucleotide excision repair |
| Havana BLAST/BLAT: |
OTTHUMG00000168209 |
| Locus Specific Databases: |
LRG_474 |