| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Plectin |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
1-0, 5-1ug/ml, 5ug/ml, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Optimal dilution of the Plectin antibody should be determined by the researcher |
| Intented use: |
This Plectin antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q15149 |
| Purity: |
Antigen affinity |
| Description: |
0a, 1j and 1h have not been confirmed in human, Analysis of the human locus has identified eight of the eleven alternative 5' exons found in mouse and rat, As the expression of specific plectin isoforms was found to be dependent on cell type (tissue) and stage of development, Concordantly, Eleven exons (1-1j) have been identified that alternatively splice directly into a common exon 2 which is the first exon to encode plectin's highly conserved actin binding domain (ABD), Furthermore, Plakins, Plectin is expressed as several protein isoforms in a wide range of cell types and tissues from a single gene located on chromosome 8 in humans, The plectin gene locus in mouse on chromosome 15 has been analyzed in detail, The short alternative amino-terminal sequences encoded by the different first exons direct the targeting of the various isoforms to distinct subcellular locations, Three additional exons (-1, and 0) splice into an alternative first coding exon (1c), and human, and regulation of signaling complexes, and two additional exons (2alpha and 3alpha) are optionally spliced within the exons encoding the acting binding domain (exons 2-8), as if custom-made for specific requirements of the particular cells, but also serve as scaffolding platforms for the assembly, exons 1i, individual isoforms were found to carry out distinct and specific functions, is a prominent member of an important family of structurally and in part functionally related proteins, isoforms lacking the central rod domain encoded by exon 31 have been detected in mouse, it appears that each cell type (tissue) contains a unique set (proportion and composition) of plectin isoforms, known as PLEC, not only play crucial roles in maintaining cell and tissue integrity and orchestrating dynamic changes in cytoarchitecture and cell shape, positioning, rat, revealing a genomic exon-intron organization with well over 40 exons spanning over 62 kb and an unusual 5' transcript complexity of plectin isoforms, termed plakins or cytolinkers, that are capable of interlinking different elements of the cytoskeleton, with their multi-domain structure and enormous size, Plectin |
| Immunogen: |
Amino acids RFIEQEKAKLEQLFQDEVAKAQQLREEQ of human Plectin were used as the immunogen for the Plectin antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the Plectin antibody may be kept for up to one month refrigerated at +4 degrees C, After reconstitution, For long-term |
| Localization: |
cytoskeletal, Cytoplasmic |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Plectin |
| Short name: |
Plectin Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
Plectin (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
9069 |
| Gene: |
PLEC |
More about : PLEC |
| Long gene name: |
plectin |
| Synonyms gene: |
EBS1 PLEC1 |
| Synonyms gene name: |
500kD epidermolysis bullosa simplex 1 (Ogna) plectin 1, intermediate filament binding protein, intermediate filament binding protein 500kDa , plectin 1 |
| Synonyms: |
PCN PLTN |
| Locus: |
8q24, 3 |
| Discovery year: |
1997-08-22 |
| GenBank acession: |
U53204 |
| Entrez gene record: |
5339 |
| Pubmed identfication: |
8633055 8696340 |
| RefSeq identity: |
NM_000445 |
| Classification: |
Plakins |
| Havana BLAST/BLAT: |
OTTHUMG00000165291 |