| Antigen detectable with this ELISA kit: |
Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) |
| Species reactivity: |
Rattus norvegicus (Rat) |
| Range of detection: |
0, 1, 10ng/mL, 2, 20ng/mL, 5ng/mL, 25ng/mL, 5ng/mL, 625-40ng/mL The standard curve concentrations used for the ELISA&rsquo, 625ng/mL, s were 40ng/mL |
| Sensitivity: |
228ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, Tissue homogenates |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3), The concentration of Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) |
| Short name: |
Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
B-cell CLL/lymphoma 2/Adenovirus E1B 19kDa Interacting Protein 3 (B-cell CLL/lymphoma 2/adenovirus E1B 19kDa interacting protein 3) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
BNIP3 and IDBG-641206 and ENSBTAG00000017804 and 615342, BNIP3 and IDBG-93314 and ENSG00000176171 and 664, Bnip3 and IDBG-269444 and ENSMUSG00000078566 and 12176, GTPase binding, NIP3, nuclei, this GO :0001666 and response to hypoxia and biological process this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005635 and nuclear envelope and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005739 and mitochondrion and cellular component this GO :0005740 and mitochondrial envelope and cellular component this GO :0005741 and mitochondrial outer membrane and cellular component this GO :0005783 and endoplasmic reticulum and cellular component this GO :0006915 and apoptotic process and biological process this GO :0008219 and cell death and biological process this GO :0008626 and granzyme-mediated apoptotic signaling pathway and biological process this GO :0010508 and positive regulation of autophagy and biological process this GO :0010637 and negative regulation of mitochondrial fusion and biological process this GO :0016021 and integral component of membrane and cellular component this GO :0016032 and viral process and biological process this GO :0030425 and dendrite and cellular component this GO :0031307 and integral component of mitochondrial outer membrane and cellular component this GO :0031966 and mitochondrial membrane and cellular component this GO :0035694 and mitochondrial protein catabolic process and biological process this GO :0042802 and identical protein binding and molecular function this GO :0042803 and protein homodimerization activity and molecular function this GO :0043065 and positive regulation of apoptotic process and biological process this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0043068 and positive regulation of programmed cell death and biological process this GO :0043243 and positive regulation of protein complex disassembly and biological process this GO :0043653 and mitochondrial fragmentation involved in apoptotic process and biological process this GO :0045837 and negative regulation of membrane potential and biological process this GO :0046902 and regulation of mitochondrial membrane permeability and biological process this GO :0046982 and protein heterodimerization activity and molecular function this GO :0048102 and autophagic cell death and biological process this GO :0050873 and brown fat cell differentiation and biological process this GO :0051020 and GTPase binding and molecular function this GO :0051402 and neuron apoptotic process and biological process this GO :0051607 and defense response to virus and biological process this GO :0055093 and response to hyperoxia and biological process this GO :0070301 and cellular response to hydrogen peroxide and biological process this GO :0071260 and cellular response to mechanical stimulus and biological process this GO :0071279 and cellular response to cobalt ion and biological process this GO :0071456 and cellular response to hypoxia and biological process this GO :0072593 and reactive oxygen species metabolic process and biological process this GO :0090141 and positive regulation of mitochondrial fission and biological process this GO :0090200 and positive regulation of release of cytochrome c from mitochondria and biological process this GO :0097193 and intrinsic apoptotic signaling pathway and biological process this GO :0097345 and mitochondrial outer membrane permeabilization and biological process this GO :1990144 and intrinsic apoptotic signaling pathway in response to hypoxia and biological process, this GO :0005515 : protein binding, this GO :0005515 : protein binding and also this GO :0042802 : identical protein binding and also this GO :0042803 : protein homodimerization activity and also this GO :0046982 : protein heterodimerization activity and also this GO :0051020 : GTPase binding, this GO :0042802 : identical protein binding, this GO :0042803 : protein homodimerization activity, this GO :0046982 : protein heterodimerization activity, this GO :0051020 : GTPase binding, BCL2/adenovirus E1B 19kDa interacting protein 3 |
| Identity: |
1084 |
| Gene: |
BNIP3 |
More about : BNIP3 |
| Long gene name: |
BCL2 interacting protein 3 |
| Synonyms gene name: |
BCL2/adenovirus E1B 19kDa interacting protein 3 |
| Synonyms: |
Nip3 |
| Locus: |
10q26, 3 |
| Discovery year: |
1997-03-19 |
| GenBank acession: |
U15174 |
| Entrez gene record: |
664 |
| Pubmed identfication: |
7954800 |
| Classification: |
BCL2 homology region 3 (BH3) only |
| Havana BLAST/BLAT: |
OTTHUMG00000019278 |