| Antigen detectable with this ELISA kit: |
Bcl2 Associated Athanogene 3 (BAG3) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
056ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, plasma, tissue homogenates, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bcl2 Associated Athanogene 3 (BAG3), The concentration of Bcl2 Associated Athanogene 3 (BAG3) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bcl2 Associated Athanogene 3 (BAG3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Bcl2 Associated Athanogene 3 (BAG3), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Bcl2 Associated Athanogene 3 (BAG3) |
| Short name: |
Bcl2 Associated Athanogene 3 (BAG3) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
B-cell CLL/lymphoma 2 Associated Athanogene 3 (B-cell CLL/lymphoma 2-associated athanogene 3) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
BAG-3 and BIS and CAIR-1 and MFM6, BAG3 and IDBG-91218 and ENSG00000151929 and 9531, BT, Bag3 and IDBG-210660 and ENSMUSG00000030847 and 29810, Plasma membranes, chaperone binding, this GO :0005515 and protein binding and molecular function this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006457 and protein folding and biological process this GO :0007420 and brain development and biological process this GO :0008625 and extrinsic apoptotic signaling pathway via death domain receptors and biological process this GO :0010664 and negative regulation of striated muscle cell apoptotic process and biological process this GO :0021510 and spinal cord development and biological process this GO :0030018 and Z disc and cellular component this GO :0032403 and protein complex binding and molecular function this GO :0043005 and neuron projection and cellular component this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0050821 and protein stabilization and biological process this GO :0051087 and chaperone binding and molecular function this GO :0071260 and cellular response to mechanical stimulus and biological process this GO :0097192 and extrinsic apoptotic signaling pathway in absence of ligand and biological process, this GO :0005515 : protein binding, this GO :0005515 : protein binding and also this GO :0032403 : protein complex binding and also this GO :0051087 : chaperone binding, this GO :0032403 : protein complex binding, this GO :0051087 : chaperone binding, 65578 and IDBG-640385 and ENSBTAG00000013641 and 782633, BCL2-associated athanogene 3 |
| Identity: |
939 |
| Gene: |
BAG3 |
More about : BAG3 |
| Long gene name: |
BCL2 associated athanogene 3 |
| Locus: |
10q26, 11 |
| Discovery year: |
1999-04-23 |
| GenBank acession: |
AF095193 |
| Entrez gene record: |
9531 |
| Pubmed identfication: |
9873016 18094623 |
| RefSeq identity: |
NM_004281 |
| Classification: |
BCL2 associated athanogene family |
| Havana BLAST/BLAT: |
OTTHUMG00000019155 |
| Locus Specific Databases: |
LRG_742 |