| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
CTCF |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
1-0, 5-1ug/ml, 5ug/ml, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Optimal dilution of the CTCF antibody should be determined by the researcher |
| Intented use: |
This CTCF antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P49711 |
| Purity: |
Antigen affinity |
| Description: |
Alternatively spliced transcript variants encoding different isoforms have been found for this gene, And this nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins, Depending upon the context of the site, If the protein is bound to a transcriptional insulator element, Mutations in this gene have been associated with invasive breast cancers, This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains, and Wilms' tumors, it can block communication between enhancers and upstream promoters, prostate cancers, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor, thereby regulating imprinted expression, Transcriptional repressor CTCF also known as 11-zinc finger protein or CCCTC-binding factor is a transcription factor that in humans is encoded by the CTCF gene |
| Immunogen: |
Amino acids 521-727 of human CTCF were used as the immunogen for the CTCF antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the CTCF antibody may be kept for up to one month refrigerated at +4 degrees C, After reconstitution, For long-term |
| Localization: |
Nuclear |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
CTCF |
| Short name: |
CTCF Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
CTCF (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
13723 |
| Gene: |
CTCF |
More about : CTCF |
| Long gene name: |
CCCTC-binding factor |
| Synonyms gene name: |
CCCTC-binding factor (zinc finger protein) |
| Synonyms name: |
11 zinc finger transcriptional repressor |
| Locus: |
16q22, 1 |
| Discovery year: |
2000-10-20 |
| GenBank acession: |
U25435 |
| Entrez gene record: |
10664 |
| Pubmed identfication: |
8649389 18550811 |
| RefSeq identity: |
NM_006565 |
| Classification: |
Zinc fingers C2H2-type |
| Havana BLAST/BLAT: |
OTTHUMG00000137539 |