| Long name: |
CTCF Primary Polyclonal Antibody |
| Also known as: |
CTCF PAb |
| Category: |
Primary Antibodies |
| Conjugation: |
Unconjugated |
| Target Antigen: |
CTCF |
| Specificity: |
This is a highly specific antibody against CTCF |
| Modification(s): |
None |
| Modification site(s): |
Unmodified antibody |
| Clonality: |
Polyclonal antibody |
| Clone number: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen range: |
470-520/727 |
| Subcellular location: |
Nucleus |
| Source: |
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human CTCF |
| Gene ID number: |
10664 |
| Swiss Prot: |
P49711 |
| Tested Applications: |
IF(IHC-P), IHC-P, WB |
| Recommended dilutions: |
IF(IHC-P)(1:50-200), IHC-P(1:100-500), WB(1:100-1000) |
| Cross reactivity: |
Mouse, Rat, Human |
| Cross reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the target antigen: |
Acts as a transcriptional activator of APP, Acts as transcriptional repressor binding to promoters of vertebrate MYC gene and BAG1 gene, Also binds to the PLK and PIM1 promoters, Can dimerize when it is bound to different DNA sequences, Causes local loss of histone acetylation and gain of histone methylation in the beta-globin locus, Inversely, Involved in transcriptional regulation by binding to chromatin insulators and preventing interaction between promoter and nearby enhancers and silencers, May participate with Tsix in establishing a regulatable epigenetic switch for X chromosome inactivation, Mediates interchromosomal association between IGF2/H19 and WSB1/NF1 and may direct distant DNA segments to a common transcription factory, On the maternal allele, Participates in the allele-specific gene expression at the imprinted IGF2/H19 gene locus, Plays a critical role in gene silencing over considerable distances in the genome, Plays a critical role in the epigenetic regulation, Plays a important role in chromatin remodeling, Plays an essential role in oocyte and preimplantation embryo development by activating or repressing transcription, Preferentially interacts with unmethylated DNA, Regulates APOA1/C3/A4/A5 gene cluster and controls MHC class II gene expression, Seems to act as tumor suppressor, Seems to be essential for homologous X-chromosome pairing, When bound to chromatin, binding to target sites is prevented by CpG methylation, binding within the H19 imprinting control region (ICR) mediates maternally inherited higher-order chromatin conformation to restrict enhancer access to IGF2, it provides an anchor point for nucleosomes positioning, mediating long-range chromatin looping, preventing spreading of CpG methylation and maintaining methylation-free zones, without affecting transcription, Chromatin binding factor that binds to DNA sequence specific sites |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA |
| Synonym names: |
11-zinc finger protein, CCCTC-binding factor, CTCF, CTCFL paralog, Transcriptional repressor CTCF, MRD21 |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
CTCF |
| Short name: |
CTCF Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
CTCF (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
13723 |
| Gene: |
CTCF |
More about : CTCF |
| Long gene name: |
CCCTC-binding factor |
| Synonyms gene name: |
CCCTC-binding factor (zinc finger protein) |
| Synonyms name: |
11 zinc finger transcriptional repressor |
| Locus: |
16q22, 1 |
| Discovery year: |
2000-10-20 |
| GenBank acession: |
U25435 |
| Entrez gene record: |
10664 |
| Pubmed identfication: |
8649389 18550811 |
| RefSeq identity: |
NM_006565 |
| Classification: |
Zinc fingers C2H2-type |
| Havana BLAST/BLAT: |
OTTHUMG00000137539 |