| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
RAGE |
| Specificity: |
This antibody reacts specifically with RAGE |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Extracellular |
| Antigen Source: |
KLH conjugated synthetic peptide derived from rat AGER |
| Gene ID: |
81722 |
| Swiss Prot: |
Q63495 |
| Applications: |
IF(IHC-P), FCM |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200), FCM(1:20-100) |
| Cross reactive species: |
Rat (Rattus norvegicus) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
ABPP-initiated RAGE signaling, AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes, Can also bind oligonucleotides (By similarity), Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons, Interaction with S1A12 on endothelium, Interaction with S1B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling, Receptor for amyloid beta peptide, These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes, and endothelial dysfunction in type 2 diabetes, and lymphocytes triggers cellular activation, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space, mononuclear phagocytes, oxidative stress, with generation of key proinflammatory mediators, Mediates interactions of advanced glycosylation end products (AGE) |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
RAGE SCARJ1 |
| Also known as: |
RAGE Polyclonal Antibody |
| Other name: |
Anti-RAGE Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
RAGE |
| Short name: |
Anti-RAGE (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-RAGE (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
320 |
| Gene: |
AGER |
More about : AGER |
| Long gene name: |
advanced glycosylation end-product specific receptor |
| Synonyms name: |
receptor for advanced glycation end-products |
| Locus: |
6p21, 32 |
| Discovery year: |
1994-10-17 |
| GenBank acession: |
M91211 |
| Entrez gene record: |
177 |
| Pubmed identfication: |
7713518 |
| RefSeq identity: |
NM_001136 |
| Classification: |
C2-set domain containing Immunoglobulin like domain containing Scavenger receptors |
| Havana BLAST/BLAT: |
OTTHUMG00000031120 |