| Antigen detectable with this ELISA kit: |
Aurora Kinase B (AURKB) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
055ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
Tissue homogenates and other biological fluids |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aurora Kinase B (AURKB), The concentration of Aurora Kinase B (AURKB) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aurora Kinase B (AURKB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Aurora Kinase B (AURKB), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Aurora Kinase B (AURKB) |
| Short name: |
Aurora Kinase B (AURKB) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Aurora phosphorylation catalyst B (aurora kinase B) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
AIK2 and AIM-1 and AIM1 and ARK2 and AurB and aurkb-sv1 and aurkb-sv2 and IPL1 and PPP1R48 and STK12 and STK5, AURKB and IDBG-28278 and ENSG00000178999 and 9212, AURKB and IDBG-635657 and ENSBTAG00000001717 and 360192, Aurkb and IDBG-189620 and ENSMUSG00000020897 and 20877, centromeric region and cellular component this GO :0000780 and condensed nuclear chromosome, centromeric region and cellular component this GO :0002903 and negative regulation of B cell apoptotic process and biological process this GO :0004672 and protein kinase activity and molecular function this GO :0004674 and protein serine/threonine kinase activity and molecular function this GO :0004712 and protein serine/threonine/tyrosine kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005819 and spindle and cellular component this GO :0005829 and cytosol and cellular component this GO :0006468 and protein phosphorylation and biological process this GO :0007067 and mitotic nuclear division and biological process this GO :0007568 and aging and biological process this GO :0008283 and cell proliferation and biological process this GO :0008608 and attachment of spindle microtubules to kinetochore and biological process this GO :0009838 and abscission and biological process this GO :0010369 and chromocenter and cellular component this GO :0016570 and histone modification and biological process this GO :0016772 and transferase activity, nuclei, this GO :0000122 and negative regulation of transcription from RNA polymerase II promoter and biological process this GO :0000278 and mitotic cell cycle and biological process this GO :0000779 and condensed chromosome, this GO :0004672 : protein kinase activity, this GO :0004672 : protein kinase activity and also this GO :0004674 : protein serine/threonine kinase activity and also this GO :0004712 : protein serine/threonine/tyrosine kinase activity and also this GO :0004713 : protein tyrosine kinase activity and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0016772 : transferase activity, this GO :0004674 : protein serine/threonine kinase activity, this GO :0004712 : protein serine/threonine/tyrosine kinase activity, this GO :0004713 : protein tyrosine kinase activity, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0016772 : transferase activity, this GO :0035174 : histone serine kinase activity, this GO :0046872 : metal ion binding, transferase activity, transferring phosphorus-containing groups, transferring phosphorus-containing groups and also this GO :0035174 : histone serine kinase activity and also this GO :0046872 : metal ion binding, transferring phosphorus-containing groups and molecular function this GO :0030496 and midbody and cellular component this GO :0031145 and anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process and biological process this GO :0031565 and cytokinesis checkpoint and biological process this GO :0031577 and spindle checkpoint and biological process this GO :0032091 and negative regulation of protein binding and biological process this GO :0032133 and chromosome passenger complex and cellular component this GO :0032466 and negative regulation of cytokinesis and biological process this GO :0032467 and positive regulation of cytokinesis and biological process this GO :0034501 and protein localization to kinetochore and biological process this GO :0034644 and cellular response to UV and biological process this GO :0035174 and histone serine kinase activity and molecular function this GO :0036089 and cleavage furrow formation and biological process this GO :0043146 and spindle stabilization and biological process this GO :0043988 and histone H3-S28 phosphorylation and biological process this GO :0045171 and intercellular bridge and cellular component this GO :0046777 and protein autophosphorylation and biological process this GO :0046872 and metal ion binding and molecular function this GO :0051256 and spindle midzone assembly involved in mitosis and biological process this GO :0051983 and regulation of chromosome segregation and biological process, aurora kinase B |
| Identity: |
11390 |
| Gene: |
AURKB |
More about : AURKB |
| Long gene name: |
aurora kinase B |
| Synonyms gene: |
STK12 |
| Synonyms gene name: |
serine/threonine kinase 12 |
| Synonyms: |
Aik2 IPL1 AurB AIM-1 ARK2 STK5 PPP1R48 |
| Synonyms name: |
aurora-B aurora-1 protein phosphatase 1, regulatory subunit 48 |
| Locus: |
17p13, 1 |
| Discovery year: |
1999-01-29 |
| GenBank acession: |
AF004022 |
| Entrez gene record: |
9212 |
| Pubmed identfication: |
9858806 |
| RefSeq identity: |
NM_004217 |
| Classification: |
Protein phosphatase 1 regulatory subunits Chromosomal passenger complex |
| Havana BLAST/BLAT: |
OTTHUMG00000108189 |