| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
CYP7A1 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
1-0, 5ug/ml, Western blot: 0 |
| Notes: |
Optimal dilution of the CYP7A1 antibody should be determined by the researcher |
| Intented use: |
This CYP7A1 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P22680 |
| Purity: |
Antigen affinity |
| Description: |
(1992) identified areas of the CYP7A1 promoter region that showed hepatocyte-specific activation, (1992) mapped the CYP7 gene to 8q11-q12, (2002) found that wildtype mice and mice transgenic for human CYP7A1 respond differently to cholesterol feeding, Agellon et al, By transfection of reporter constructs, Cholesterol feeding stimulated Cyp7a1 mRNA abundance and enzymatic activity in wildtype mice, Cohen et al, Molowa et al, They found HNF3 to be an activator of CYP7A1 activity, Using both mouse-human somatic cell hybrids and in situ chromosomal hybridization, also called CYP7, and DNase footprinting, but repressed human CYP7A1 mRNA and activity in transgenic mice, is an enzyme that in humans is encoded by the CYP7A1 gene, mutation analysis, CYP7A1 (Cytochrome P450 Subfamily VIIA Polypeptide 1) |
| Immunogen: |
Amino acids 301-504 of human CYP7A1 were used as the immunogen for the CYP7A1 antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the CYP7A1 antibody may be kept for up to one month refrigerated at +4 degrees C, After reconstitution, For long-term |
| Localization: |
membranous, Cytoplasmic |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
CYP7A1 |
| Short name: |
CYP7A1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
CYP7A1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
2651 |
| Gene: |
CYP7A1 |
More about : CYP7A1 |
| Long gene name: |
cytochrome P450 family 7 subfamily A member 1 |
| Synonyms gene: |
CYP7 |
| Synonyms gene name: |
cytochrome P450, family 7, polypeptide 1 , polypeptide 1 cytochrome P450, subfamily A, subfamily VIIA (cholesterol 7 alpha-monooxygenase) |
| Synonyms name: |
cholesterol 7 alpha-monooxygenase |
| Locus: |
8q12, 1 |
| Discovery year: |
1992-04-16 |
| GenBank acession: |
M89803 |
| Entrez gene record: |
1581 |
| Pubmed identfication: |
1358792 |
| RefSeq identity: |
NM_000780 |
| Classification: |
Cytochrome P450 family 7 |
| Havana BLAST/BLAT: |
OTTHUMG00000164301 |