| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
DFFB |
| Specificity: |
This antibody reacts specifically with DFFB |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus, Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from mouse DFF-40 beta |
| Gene ID: |
13368 |
| Swiss Prot: |
O54788 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Rat (Rattus norvegicus), Mouse (Mus musculus) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Degrades naked DNA and induces apoptotic morphology, Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
CAD CPAN DFF-40 DFF40 |
| Also known as: |
DFFB Polyclonal Antibody |
| Other name: |
Anti-DFFB Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
DFFB |
| Short name: |
Anti-DFFB (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
40kDa, ALEXA Fluor 594, beta polypeptide (caspase-activated DNase) (polyclonal), antibody to-Desoxyribonucleic acid fragmentation factor |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
40kDa, BT, CAD and CPAN and DFF-40 and DFF2 and DFF40, DFFB and IDBG-86952 and ENSG00000169598 and 1677, Dffb and IDBG-206807 and ENSMUSG00000029027 and 13368, beta polypeptide (caspase-activated DNase), enzyme binding, nuclei, this GO :0000790 and nuclear chromatin and cellular component this GO :0003677 and DNA binding and molecular function this GO :0004518 and nuclease activity and molecular function this GO :0004536 and deoxyribonuclease activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005622 and intracellular and cellular component this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0006309 and apoptotic DNA fragmentation and biological process this GO :0006915 and apoptotic process and biological process this GO :0006921 and cellular component disassembly involved in execution phase of apoptosis and biological process this GO :0007420 and brain development and biological process this GO :0016787 and hydrolase activity and molecular function this GO :0019899 and enzyme binding and molecular function this GO :0030263 and apoptotic chromosome condensation and biological process, this GO :0003677 : DNA binding, this GO :0003677 : DNA binding and also this GO :0004518 : nuclease activity and also this GO :0004536 : deoxyribonuclease activity and also this GO :0005515 : protein binding and also this GO :0016787 : hydrolase activity and also this GO :0019899 : enzyme binding, this GO :0004518 : nuclease activity, this GO :0004536 : deoxyribonuclease activity, this GO :0005515 : protein binding, this GO :0016787 : hydrolase activity, this GO :0019899 : enzyme binding, 22809 and IDBG-634397 and ENSBTAG00000020015 and 512730, DNA fragmentation factor |
| Identity: |
2773 |
| Gene: |
DFFB |
More about : DFFB |
| Long gene name: |
DNA fragmentation factor subunit beta |
| Synonyms gene name: |
40kDa, DNA fragmentation factor, beta polypeptide (caspase-activated DNase) |
| Synonyms name: |
caspase-activated DNase |
| Locus: |
1p36, 32 |
| Discovery year: |
1997-10-27 |
| Entrez gene record: |
1677 |
| Pubmed identfication: |
9108473 9560346 |
| RefSeq identity: |
NM_001282669 |
| Havana BLAST/BLAT: |
OTTHUMG00000003525 |