| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
ERK1 + ERK2 |
| Specificity: |
This antibody reacts specifically with ERK1 + ERK2 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus, Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from mouse ERK2 |
| Gene ID: |
26413 |
| Swiss Prot: |
P63085 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
About 160 substrates have already been discovered for ERKs, Depending on the cellular context, However, MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade, Many of these substrates are localized in the nucleus, Moreover, The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF, adhesion, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors, and seem to participate in the regulation of transcription upon stimulation, and those are responsible for processes such as translation, as well as in the fragmentation of the Golgi apparatus during mitosis, cytoskeletal rearrangements, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), mitosis, mitosis and apoptosis, other substrates are found in the cytosol as well as in other cellular organelles, survival and differentiation through the regulation of transcription, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, translation, Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
ERK ERK2 p41mapk MAPK2 |
| Also known as: |
ERK1 + ERK2 Polyclonal Antibody |
| Other name: |
Anti-ERK1 + ERK2 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
ERK1 + ERK2 |
| Short name: |
Anti-ERK1 + ERK2 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-ERK1 and ERK2 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
6871 |
| Gene: |
MAPK1 |
More about : MAPK1 |
| Long gene name: |
mitogen-activated protein kinase 1 |
| Synonyms gene: |
PRKM2 PRKM1 |
| Locus: |
22q11, 22 |
| Discovery year: |
1993-11-05 |
| GenBank acession: |
M84489 |
| Entrez gene record: |
5594 |
| Classification: |
Mitogen-activated protein kinases |
| Havana BLAST/BLAT: |
OTTHUMG00000030508 |
| Locus Specific Databases: |
LRG_786 |