| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
DNA polymerase beta / POLB |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
1-0, 5ug/ml, Western blot: 0 |
| Notes: |
Optimal dilution of the DNA polymerase beta antibody should be determined by the researcher |
| Intented use: |
This DNA polymerase beta antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P06746 |
| Purity: |
Antigen affinity |
| Description: |
Additionally, In vitro, It is found that a truncated POLB is expressed in primary colorectal tumors and inhibits the normal repair function of wildtype POLB, It is localized on 8p11, Several transcript variants of this gene exist, The encoded protein, The increase in DNA polymerase activity upon methylation is due to the enhanced DNA binding and processivity of POLB, The protein encoded by this gene is a DNA polymerase involved in base excision and repair, acting as a monomer, also called gap-filling DNA synthesis, also known as POLB, beta, but it translocates to the nucleus upon DNA damage, but the full-length nature of only one has been described to date, human POLB forms a complex with and is methylated by PRMT6, in humans, is an enzyme that, is encoded by the POLB gene, is normally found in the cytoplasm, methylated POLB possesses significantly higher DNA polymerase activity when compared to that of unmodified enzyme, 2, Polymerase (DNA directed) |
| Immunogen: |
Amino acids 122-335 of human POLB were used as the immunogen for the DNA polymerase beta antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the DNA polymerase beta antibody may be kept for up to one month refrigerated at +4 degrees C, After reconstitution, For long-term |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
DNA polymerase beta / POLB |
| Short name: |
DNA polymerase beta Antibody / POLB |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
beta, Desoxyribonucleic acid polymerizing enzyme b (Antibody to) / polymerase (Desoxyribonucleic acid directed) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
POLB and IDBG-20094 and ENSG00000070501 and 102723810, POLB and IDBG-629853 and ENSBTAG00000000225 and 614688, Polb and IDBG-140909 and ENSMUSG00000031536 and 18970, beta, gap-filling and biological process this GO :0006290 and pyrimidine dimer repair and biological process this GO :0006974 and cellular response to DNA damage stimulus and biological process this GO :0008017 and microtubule binding and molecular function this GO :0008152 and metabolic process and biological process this GO :0008630 and intrinsic apoptotic signaling pathway in response to DNA damage and biological process this GO :0016779 and nucleotidyltransferase activity and molecular function this GO :0016829 and lyase activity and molecular function this GO :0019899 and enzyme binding and molecular function this GO :0034061 and DNA polymerase activity and molecular function this GO :0045471 and response to ethanol and biological process this GO :0046872 and metal ion binding and molecular function this GO :0051402 and neuron apoptotic process and biological process this GO :0071897 and DNA biosynthetic process and biological process, metal ion binding, nuclei, this GO :0003677 and DNA binding and molecular function this GO :0003684 and damaged DNA binding and molecular function this GO :0003887 and DNA-directed DNA polymerase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005874 and microtubule and cellular component this GO :0005876 and spindle microtubule and cellular component this GO :0006261 and DNA-dependent DNA replication and biological process this GO :0006281 and DNA repair and biological process this GO :0006284 and base-excision repair and biological process this GO :0006287 and base-excision repair, this GO :0003677 : DNA binding, this GO :0003677 : DNA binding and also this GO :0003684 : damaged DNA binding and also this GO :0003887 : DNA-directed DNA polymerase activity and also this GO :0005515 : protein binding and also this GO :0008017 : microtubule binding and also this GO :0016779 : nucleotidyltransferase activity and also this GO :0016829 : lyase activity and also this GO :0019899 : enzyme binding and also this GO :0034061 : DNA polymerase activity and also this GO :0046872 : metal ion binding, this GO :0003684 : damaged DNA binding, this GO :0003887 : DNA-directed DNA polymerase activity, this GO :0005515 : protein binding, this GO :0008017 : microtubule binding, this GO :0016779 : nucleotidyltransferase activity, this GO :0016829 : lyase activity, this GO :0019899 : enzyme binding, this GO :0034061 : DNA polymerase activity, this GO :0046872 : metal ion binding, 5423, polymerase (DNA directed) |
| Identity: |
9174 |
| Gene: |
POLB |
More about : POLB |
| Long gene name: |
DNA polymerase beta |
| Synonyms gene name: |
beta polymerase (DNA) beta , polymerase (DNA directed) |
| Locus: |
8p11, 21 |
| Discovery year: |
2001-06-22 |
| Entrez gene record: |
5423 |
| RefSeq identity: |
NM_002690 |
| Classification: |
DNA polymerases |
| Havana BLAST/BLAT: |
OTTHUMG00000164093 |