| Antigen detectable with this ELISA kit: |
Asparagine Synthetase (ASNS) |
| Species reactivity: |
Mus musculus (Mouse) |
| Range of detection: |
0, 0, 1, 10ng/mL, 2, 5ng/mL, 25ng/mL, 313-20ng/mL The standard curve concentrations used for the ELISA&rsquo, 313ng/mL, 5ng/mL, 625ng/mL, s were 20ng/mL |
| Sensitivity: |
108ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
plasma, tissue homogenates and other biological fluids, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Asparagine Synthetase (ASNS), The concentration of Asparagine Synthetase (ASNS) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Asparagine Synthetase (ASNS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Asparagine Synthetase (ASNS), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Asparagine Synthetase (ASNS) |
| Short name: |
Asparagine Synthetase (ASNS) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Asparagine Synthetase (asparagine synthetase (glutamine-hydrolyzing)) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
ASNS and IDBG-28257 and ENSG00000070669 and 440, ASNS and IDBG-629426 and ENSBTAG00000003222 and 514209, ASNSD and TS11, Asns and IDBG-128303 and ENSMUSG00000029752 and 102641622, Cytoplasm, cofactor binding, this GO :0001889 and liver development and biological process this GO :0004066 and asparagine synthase (glutamine-hydrolyzing) activity and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005829 and cytosol and cellular component this GO :0006520 and cellular amino acid metabolic process and biological process this GO :0006529 and asparagine biosynthetic process and biological process this GO :0006541 and glutamine metabolic process and biological process this GO :0006987 and activation of signaling protein activity involved in unfolded protein response and biological process this GO :0008152 and metabolic process and biological process this GO :0008652 and cellular amino acid biosynthetic process and biological process this GO :0009416 and response to light stimulus and biological process this GO :0009612 and response to mechanical stimulus and biological process this GO :0009636 and response to toxic substance and biological process this GO :0030968 and endoplasmic reticulum unfolded protein response and biological process this GO :0031427 and response to methotrexate and biological process this GO :0031667 and response to nutrient levels and biological process this GO :0032354 and response to follicle-stimulating hormone and biological process this GO :0032870 and cellular response to hormone stimulus and biological process this GO :0034641 and cellular nitrogen compound metabolic process and biological process this GO :0042149 and cellular response to glucose starvation and biological process this GO :0042803 and protein homodimerization activity and molecular function this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0043200 and response to amino acid and biological process this GO :0044267 and cellular protein metabolic process and biological process this GO :0044281 and small molecule metabolic process and biological process this GO :0045931 and positive regulation of mitotic cell cycle and biological process this GO :0048037 and cofactor binding and molecular function this GO :0070981 and L-asparagine biosynthetic process and biological process, this GO :0004066 : asparagine synthase (glutamine-hydrolyzing) activity, this GO :0004066 : asparagine synthase (glutamine-hydrolyzing) activity and also this GO :0005524 : ATP binding and also this GO :0042803 : protein homodimerization activity and also this GO :0048037 : cofactor binding, this GO :0005524 : ATP binding, this GO :0042803 : protein homodimerization activity, this GO :0048037 : cofactor binding, 27053, asparagine synthetase (glutamine-hydrolyzing) |
| Identity: |
753 |
| Gene: |
ASNS |
More about : ASNS |
| Long gene name: |
asparagine synthetase (glutamine-hydrolyzing) |
| Synonyms gene name: |
asparagine synthetase |
| Locus: |
7q21, 3 |
| Discovery year: |
2001-06-22 |
| GenBank acession: |
M27396 |
| Entrez gene record: |
440 |
| RefSeq identity: |
NM_001673 NM_183356 |
| Havana BLAST/BLAT: |
OTTHUMG00000022892 |