| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
DHFR |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Titration of the DHFR antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This DHFR antibodyis to be used only for research purposes and not for diagnostics |
| Gene ID #: |
1719 |
| Purity: |
Antigen affinity |
| Description: |
DHFR belongs to the dihydrofolate reductase family, DHFR catalyzes an essential reaction for de novo glycine and purine synthesis, In addition, It is the key enzyme in folate metabolism, a methyl group shuttle required for the de novo synthesis of purines, and certain amino acids, and for DNA precursor synthesis, and it converts dihydrofolate into tetrahydrofolate, is an enzyme that reduces dihydrofolic acid to tetrahydrofolic acid using NADPH as electron donor, or DHFR, thymidylic acid, which can be converted to the kinds of tetrahydrofolate cofactors used in 1-carbon transfer chemistry, Dihydrofolate reductase |
| Immunogen: |
Human partial recombinant protein (AA 2-187) was used as the immunogen for this DHFR antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the DHFR antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
DHFR |
| Short name: |
DHFR Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
DHFR (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
2861 |
| Gene: |
DHFR |
More about : DHFR |
| Long gene name: |
dihydrofolate reductase |
| Locus: |
5q14, 1 |
| Discovery year: |
2001-06-22 |
| Entrez gene record: |
1719 |
| RefSeq identity: |
NM_000791 |
| Havana BLAST/BLAT: |
OTTHUMG00000162529 |