| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Gli1 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Titration of the Gli1 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This Gli1 antibodyis to be used only for research purposes and not for diagnostics |
| Gene ID #: |
2735 |
| Purity: |
Antigen affinity |
| Description: |
Beside, GLI1 encodes a member of the GLI-Kruppel family of zinc finger proteins in the final steps of Hedgehog signaling in normal development and disease, The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop, The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation, The protein is encoded by GLI1 gene, This gene is a member of a select group of cellular genes that are genetically altered in primary human tumors, also known as Gli, is a protein originally isolated in human glioblastoma, various upstream patterning signals may be integrated by the Gli proteins to direct a coherent programme of neurogenesis, Gli1 |
| Immunogen: |
Human partial recombinant protein (AA 768-1106) was used as the immunogen for this Gli1 antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the Gli1 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Gli1 |
| Short name: |
Gli1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
Gli1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
4317 |
| Gene: |
GLI1 |
More about : GLI1 |
| Long gene name: |
GLI family zinc finger 1 |
| Synonyms gene: |
GLI |
| Synonyms gene name: |
glioma-associated oncogene homolog 1 (zinc finger protein) glioma-associated oncogene family zinc finger 1 |
| Locus: |
12q13, 3 |
| Discovery year: |
1986-01-01 |
| Entrez gene record: |
2735 |
| Pubmed identfication: |
2850480 |
| RefSeq identity: |
NM_005269 |
| Classification: |
Zinc fingers C2H2-type |
| Havana BLAST/BLAT: |
OTTHUMG00000167332 |