| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Huntingtin |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Notes: |
Titration of the Huntingtin antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This Huntingtin antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P42858 |
| Purity: |
Antigen affinity |
| Description: |
Additionally, HTT is involved in vesicle trafficking as it interacts with HIP1, HTT upregulates the expression of Brain Derived Neurotrophic Factor (BDNF) at the transcription level, HTT was also visualized as punctate staining likely to represent nerve endings, The protein has no sequence homology with other proteins and is highly expressed in neurons and tests in humans and rodents, a clathrin-binding protein, also called HTT or HD (Huntington disease), and this gene is required for normal development, is the IT15 (interesting transcript 15) gene which codes for a protein called the Huntingtin, the absorption of materials into a cell, to mediate endocytosis, wildtype Huntingtin may function in the nucleus in the assembly of nuclear matrix-bound protein complexes involved with transcriptional repression and RNA processing, The Huntingtin gene |
| Immunogen: |
An amino acid sequence from the C-terminus of human HTT (ELDRRAFQSVLEVVAA) was used as the immunogen for this Huntingtin antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the Huntingtin antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Huntingtin |
| Short name: |
Huntingtin Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
Huntingtin (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
4851 |
| Gene: |
HTT |
More about : HTT |
| Long gene name: |
huntingtin |
| Synonyms gene: |
HD |
| Synonyms gene name: |
huntingtin (Huntington disease) |
| Synonyms: |
IT15 |
| Locus: |
4p16, 3 |
| Discovery year: |
1986-01-01 |
| GenBank acession: |
L12392 |
| Entrez gene record: |
3064 |
| Pubmed identfication: |
8458085 |
| RefSeq identity: |
NM_002111 |
| Classification: |
Endogenous ligands |
| Havana BLAST/BLAT: |
OTTHUMG00000159916 |
| Locus Specific Databases: |
LOVD - Leiden Open Variation Database LOVD Mendelian genes - huntingin (HTT) LRG_763 |