| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Securin |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Notes: |
Titration of the Securin antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This Securin antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
O95997 |
| Purity: |
Antigen affinity |
| Description: |
It also contains a destruction box(D box) that is required for its degradation by the APC, It is an anaphase-promoting complex(APC) substrate that associates with a separin until activation of the APC, It is mainly a cytosolic protein, Securin contains 2 PXXP motifs, The acidic C-terminal region of the encoded protein can act as a transactivation domain, The encoded protein is a homolog of yeast securin proteins, The gene product has transforming activity in vitro and tumorigenic activity in vivo, also known as pituitary tumor-transforming 1 or EAP1 is a protein that in humans is encoded by the PTTG1 gene, although it partially localizes in the nucleus, and is highly expressed in various tumors, as well as for its stimulation of basic fibroblast growth factor expression, which are required for its transforming and tumorigenic activities, which prevent separins from promoting sister chromatid separation, Securin |
| Immunogen: |
Amino acids 146-161 (LMILDEERELEKLFQL-human) were used as the immunogen for this Securin antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the Securin antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Securin |
| Short name: |
Securin Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
Securin (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
12427 |
| Gene: |
TUTR1 |
More about : TUTR1 |
| Long gene name: |
pituitary tumor-transforming 1 |
| Synonyms: |
PTTG HPTTG EAP1 securin PTTG1 |
| Synonyms name: |
ESP1-associated protein 1 tumor-transforming protein 1 |
| Locus: |
5q33, 3 |
| Discovery year: |
1999-02-23 |
| GenBank acession: |
AF062649 |
| Entrez gene record: |
9232 |
| Pubmed identfication: |
9811450 9892021 |
| RefSeq identity: |
NM_004219 |
| Havana BLAST/BLAT: |
OTTHUMG00000130328 |