| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
RIP |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Notes: |
Titration of the RIP antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This RIP antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q13546 |
| Purity: |
Antigen affinity |
| Description: |
By yeast 2-hybrid and coimmunoprecipitation studies using mammalian cell extracts, Hartz(2012) mapped the RIPK1 gene to chromosome 6p25, Hsu et al, Members of the TRAF protein family have been implicated in the activation of NF-kappa-B by the TNF superfamily, Stanger et al, TRAF1, TRAF2, They suggested that RIP may be an important element in the signal transduction machinery that mediates programmed cell death, also called RIP, and TRAF3, is an enzyme that in humans is encoded by the RIPK1 gene, (1995) found that overexpression of Rip in mammalian cells induced morphologic changes characteristic of apoptosis, (1996) showed that RIP interacts with TRADD, 2 based on an alignment of the RIP sequence with the genomic sequence, Receptor-interacting serine/threonine-protein kinase 1 (RIPK1) |
| Immunogen: |
An amino acid sequence from the middle region of human Receptor-interacting serine/threonine-protein kinase 1 (RRRRVSHDPFAQQRP) was used as the immunogen for this RIP antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the RIP antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
RIP |
| Short name: |
RIP Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
RIP (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
10019 |
| Gene: |
RIPK1 |
More about : RIPK1 |
| Long gene name: |
receptor interacting serine/threonine kinase 1 |
| Synonyms gene name: |
receptor (TNFRSF)-interacting serine-threonine kinase 1 |
| Synonyms: |
RIP |
| Synonyms name: |
receptor-interacting protein kinase 1 |
| Locus: |
6p25, 2 |
| Discovery year: |
1999-05-07 |
| GenBank acession: |
U25994 |
| Entrez gene record: |
8737 |
| Pubmed identfication: |
7538908 8612133 |
| RefSeq identity: |
NM_003804 |
| Classification: |
Ripoptosome |
| Havana BLAST/BLAT: |
OTTHUMG00000014134 |