| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
PAD4 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Notes: |
Titration of the PAD4 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This PAD4 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q9UM07 |
| Purity: |
Antigen affinity |
| Description: |
1999), Cuthbert et al, Deimination by PAD4 prevented arginine methylation by CARM1, Dimethylation of arginines prevented deimination by PAD4, In vivo targeting experiments on an endogenous promoter demonstrated that the protein could repress hormone receptor-mediated gene induction, It was recruited to the pS2 promoter following hormone induction when the gene was transcriptionally downregulated, Peptidylarginine deiminases make up a family of posttranslational modification enzymes that convert arginine residues to citrulline residues in the presence of calcium ion(Nakashima et al, Suzuki et al, They showed that the protein specifically deiminated arg2, Type IV is a human protein which in humans is encoded by the PADI4 gene, although monomethylation still allowed deimination to take place, and arg26 in the H3 tail, arg17, arg8, consistent with a repressive role, deimination, that converts histone arginine to citrulline and antagonizes arginine methylation, (2003) found from sequence data that the gene maps to 1p36, (2004) described a process, Peptidylarginine Deiminase |
| Immunogen: |
An amino acid sequence from the middle region of human PAD4 (ASPRSCYKLFQEQQNEGHGEA) was used as the immunogen for this PAD4 antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the PAD4 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
PAD4 |
| Short name: |
PAD4 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
PAD4 (Antibody to) |
| Alternative technique: |
antibodies |