| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Flotillin 1 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Notes: |
Titration of the Flotillin 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This Flotillin 1 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
O75955 |
| Purity: |
Antigen affinity |
| Description: |
Bickel et al, Flotillin1 colocalized with PTOV1 at the plasma membrane and in the nucleus, Hazarika et al, It consistently copurified with Flot2 and with caveolin-1 in the purification of caveolin-rich membranes, Santamaria et al, The International Radiation Hybrid Mapping Consortium mapped the gene to chromosome 6, and it entered the nucleus concomitant with PTOV1 shortly before initiation of S phase, in COS-1 cells induced filopodia formation and changed the epithelial morphology to that of neuronal cells, which they called ESA/flotillin-2, (1997) found that mouse Flot1 behaves as a resident integral membrane protein of caveolae, (1999) found that stable transfection of Flot1, (2005) found that prostate tumor overexpressed gene-1 interacted with Flotillin1 in detergent-insoluble membrane fractions, Flotillin 1 is a protein that in humans is encoded by the FLOT1 gene |
| Immunogen: |
An amino acid sequence from the middle region of human FLOT1 (KKAAYDIEVNTRRAQA) was used as the immunogen for this Flotillin 1 antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the Flotillin 1 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Flotillin 1 |
| Short name: |
Flotillin 1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
Flotillin 1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
3757 |
| Gene: |
FLOT1 |
More about : FLOT1 |
| Long gene name: |
flotillin 1 |
| Locus: |
6p21, 33 |
| Discovery year: |
2000-05-30 |
| GenBank acession: |
AF089750 |
| Entrez gene record: |
10211 |
| Classification: |
Flotillins |
| Havana BLAST/BLAT: |
OTTHUMG00000031151 |