| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
GCN2 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Notes: |
Titration of the GCN2 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This GCN2 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q9P2K8 |
| Purity: |
Antigen affinity |
| Description: |
Berlanga et al, Costa-Mattioli et al, Hartz(2005) mapped the gene to chromosome 15q15, It belongs to a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 to downregulate protein synthesis in response to varied cellular stresses, Serum starvation increased the level of phosphorylated EIF2-alpha more than 2-fold in human embryonic kidney cells transfected with mouse gcn2, a single 100-Hz train induced a strong and sustained long-term potentiation(late LTP or L-LTP), also called GCN2 (General control nonderepressible 2), is an enzyme that in humans is encoded by the EIF2AK4 gene, which was dependent on transcription and translation, (1999) demonstrated that GCN2 immunopurified from mouse liver extracts could phosphorylate rabbit Eif2 in vitro, (2005) reported a unique feature of hippocampal slices from GCN2-null mice: in CA1, 1 based on an alignment of the protein sequence with the genomic sequence, Eukaryotic Translation Initiation Factor 2-Alpha Kinase 4 |
| Immunogen: |
An amino acid sequence from the middle region of human General control nonderepressible 2 (ATDHLAFSADSKQDDQTGD) was used as the immunogen for this GCN2 antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the GCN2 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
GCN2 |
| Short name: |
GCN2 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
GCN2 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
19687 |
| Gene: |
EIF2AK4 |
More about : EIF2AK4 |
| Long gene name: |
eukaryotic translation initiation factor 2 alpha kinase 4 |
| Synonyms: |
GCN2 KIAA1338 |
| Locus: |
15q15, 1 |
| Discovery year: |
2002-11-11 |
| GenBank acession: |
AB037759 |
| Entrez gene record: |
440275 |
| Pubmed identfication: |
10504407 |
| Havana BLAST/BLAT: |
OTTHUMG00000172403 |