| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
PDK2 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ICC, IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Immunocytochemistry: 0, Western blot: 0 |
| Notes: |
Titration of the PDK2 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This PDK2 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q15119 |
| Purity: |
Antigen affinity |
| Description: |
Gudi et al, Northern blot analysis showed that the PDK2 message was found in many tissues, The human PDK2 gene encodes a protein with a predicted molecular mass of 46, To find human PDKs, and low levels in placenta and lung, intermediate levels in brain, is an enzyme that in humans is encoded by the PDK2 gene, kidney, pancreas and liver, with the highest level in heart and skeletal muscle, (1995) used oligonucleotide primers to screen a human liver cDNA library by PCR, 181 Da that shares 96% identity with that of the rat gene, Pyruvate dehydrogenase kinase isoenzyme 2 |
| Immunogen: |
mouse and rat), An amino acid sequence from the C-terminus of human PDK2 (WRHYQTIQEAGDWC) was used as the immunogen for this PDK2 antibody (100% homologous in human |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the PDK2 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
PDK2 |
| Short name: |
PDK2 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
PDK2 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
8810 |
| Gene: |
PDK2 |
More about : PDK2 |
| Long gene name: |
pyruvate dehydrogenase kinase 2 |
| Synonyms gene name: |
isoenzyme 2 pyruvate dehydrogenase kinase, isozyme 2 , pyruvate dehydrogenase kinase |
| Synonyms: |
PDHK2 |
| Locus: |
17q21, 33 |
| Discovery year: |
1996-08-14 |
| GenBank acession: |
L42451 |
| Entrez gene record: |
5164 |
| Pubmed identfication: |
7499431 |
| RefSeq identity: |
NM_002611 |
| Havana BLAST/BLAT: |
OTTHUMG00000161948 |