| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
ZEB1 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Notes: |
Titration of the ZEB1 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This ZEB1 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P37275 |
| Purity: |
Antigen affinity |
| Description: |
Fluorescence in situ hybridization localized the ZEB1 gene to chromosome 10p11, Krafchak et al, NIL2A or DELTA-EF1, Nishimura et al, They detected expression of TCF8 in cornea, also called TCF8, is a protein that in humans is encoded by the ZEB1 gene, mutant in Alport syndrome and which encodes collagen type IV alpha-3, (2005) demonstrated a complex(core plus secondary) binding site for TCF8 in the promoter of the COL4A3 gene, (2006) found that delta-Ef1 was upregulated during differentiation in a mouse smooth muscle cell(SMC) line, 2, Zinc Finger E Box-Binding Homeobox 1 |
| Immunogen: |
An amino acid sequence from the N-terminus of human Zinc Finger E Box-Binding Homeobox 1 (KDDECESDAENEQNHD) was used as the immunogen for this ZEB1 antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the ZEB1 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
ZEB1 |
| Short name: |
ZEB1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
zinc finger E-box binding homeobox 1 (Antibody to) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
AREB6 and BZP and DELTAEF1 and FECD6 and NIL2A and PPCD3 and TCF8 and ZFHEP and ZFHX1A, DNA-templated and biological process this GO :0006357 and regulation of transcription from RNA polymerase II promoter and biological process this GO :0006955 and immune response and biological process this GO :0007389 and pattern specification process and biological process this GO :0007417 and central nervous system development and biological process this GO :0008134 and transcription factor binding and molecular function this GO :0008270 and zinc ion binding and molecular function this GO :0008283 and cell proliferation and biological process this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0010464 and regulation of mesenchymal cell proliferation and biological process this GO :0014823 and response to activity and biological process this GO :0017015 and regulation of transforming growth factor beta receptor signaling pathway and biological process this GO :0030857 and negative regulation of epithelial cell differentiation and biological process this GO :0030900 and forebrain development and biological process this GO :0031667 and response to nutrient levels and biological process this GO :0033081 and regulation of T cell differentiation in thymus and biological process this GO :0043565 and sequence-specific DNA binding and molecular function this GO :0045666 and positive regulation of neuron differentiation and biological process this GO :0045892 and negative regulation of transcription, DNA-templated and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0046872 and metal ion binding and molecular function this GO :0048513 and organ development and biological process this GO :0048596 and embryonic camera-type eye morphogenesis and biological process this GO :0048598 and embryonic morphogenesis and biological process this GO :0048704 and embryonic skeletal system morphogenesis and biological process this GO :0048752 and semicircular canal morphogenesis and biological process this GO :0051150 and regulation of smooth muscle cell differentiation and biological process this GO :0051216 and cartilage development and biological process this GO :0070888 and E-box binding and molecular function this GO :0071230 and cellular response to amino acid stimulus and biological process this GO :0071560 and cellular response to transforming growth factor beta stimulus and biological process this GO :0090103 and cochlea morphogenesis and biological process, E-box binding, ZEB1 and IDBG-638511 and ENSBTAG00000020053 and 535183, ZEB1 and IDBG-66941 and ENSG00000148516 and 100996668, Zeb1 and IDBG-127909 and ENSMUSG00000024238 and 21417, nuclei, this GO :0000122 and negative regulation of transcription from RNA polymerase II promoter and biological process this GO :0003676 and nucleic acid binding and molecular function this GO :0003677 and DNA binding and molecular function this GO :0003682 and chromatin binding and molecular function this GO :0003690 and double-stranded DNA binding and molecular function this GO :0003700 and sequence-specific DNA binding transcription factor activity and molecular function this GO :0003713 and transcription coactivator activity and molecular function this GO :0003714 and transcription corepressor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005667 and transcription factor complex and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0006351 and transcription, this GO :0003676 : nucleic acid binding, this GO :0003676 : nucleic acid binding and also this GO :0003677 : DNA binding and also this GO :0003682 : chromatin binding and also this GO :0003690 : double-stranded DNA binding and also this GO :0003700 : sequence-specific DNA binding transcription factor activity and also this GO :0003713 : transcription coactivator activity and also this GO :0003714 : transcription corepressor activity and also this GO :0005515 : protein binding and also this GO :0008134 : transcription factor binding and also this GO :0008270 : zinc ion binding and also this GO :0043565 : sequence-specific DNA binding and also this GO :0046872 : metal ion binding and also this GO :0070888 : E-box binding, this GO :0003677 : DNA binding, this GO :0003682 : chromatin binding, this GO :0003690 : double-stranded DNA binding, this GO :0003700 : sequence-specific DNA binding transcription factor activity, this GO :0003713 : transcription coactivator activity, this GO :0003714 : transcription corepressor activity, this GO :0005515 : protein binding, this GO :0008134 : transcription factor binding, this GO :0008270 : zinc ion binding, this GO :0043565 : sequence-specific DNA binding, this GO :0046872 : metal ion binding, this GO :0070888 : E-box binding, 6935, zinc finger E-box binding homeobox 1 |
| Identity: |
11642 |
| Gene: |
ZEB1 |
More about : ZEB1 |
| Long gene name: |
zinc finger E-box binding homeobox 1 |
| Synonyms gene: |
TCF8 PPCD3 |
| Synonyms gene name: |
transcription factor 8 (represses interleukin 2 expression) posterior polymorphous corneal dystrophy 3 |
| Synonyms: |
BZP ZEB AREB6 NIL-2-A Zfhep Zfhx1a FECD6 |
| Locus: |
10p11, 22 |
| Discovery year: |
1991-12-17 |
| GenBank acession: |
AK091478 |
| Entrez gene record: |
6935 |
| Pubmed identfication: |
1427828 1840704 15384081 16252232 |
| RefSeq identity: |
NM_030751 |
| Classification: |
ZF class homeoboxes and pseudogenes Zinc fingers C2H2-type |
| Havana BLAST/BLAT: |
OTTHUMG00000017907 |