| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
53BP1 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ICC, IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Immunocytochemistry: 0, Western blot: 0 |
| Notes: |
Titration of the 53BP1 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting points |
| Intented use: |
This 53BP1 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q12888 |
| Purity: |
Antigen affinity |
| Description: |
G2/M checkpoint arrest, Immunoblot analysis by Iwabuchi et al, It was required for p53 accumulation, Iwabuchi et al, Iwabuchi et al, Wang et al, also called 53BP1, and the intra-S-phase checkpoint in response to ionizing radiation, is a protein that in humans is encoded by the TP53BP1 gene, (1994) showed that TP53BP1 binds to the conformationally sensitive central domain of wildtype p53 but not to mutant p53 in vitro, (1998) mapped the gene to 15q15-q21 by FISH, (1998) showed that expression of TP53BP1 or TP53BP2 enhances the transactivation function of p53 and induces the expression of p21(CDKN1A), (2002) used small interfering RNA directed against the protein in mammalian cells to demonstrate that TP53BP1 is a key transducer of the DNA damage checkpoint signal, Tumor Protein p53-Binding Protein 1 |
| Immunogen: |
An amino acid sequence from the C-terminus of human 53BP1 (ERIGFKQHPKYKHDYVSH) was used as the immunogen for this 53BP1 antibody (100% mouse homology) |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the 53BP1 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
53BP1 |
| Short name: |
53BP1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
53BP1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
11999 |
| Gene: |
TP53BP1 |
More about : TP53BP1 |
| Long gene name: |
tumor protein p53 binding protein 1 |
| Synonyms gene name: |
1 , tumor protein p53-binding protein |
| Synonyms: |
53BP1 p202 TDRD30 |
| Locus: |
15q15, 3 |
| Discovery year: |
2000-06-24 |
| GenBank acession: |
U09477 |
| Entrez gene record: |
7158 |
| Pubmed identfication: |
8016121 9748285 |
| Classification: |
Tudor domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000059757 |