| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
OSBP |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Notes: |
Titration of the OSBP antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This OSBP antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P22059 |
| Purity: |
Antigen affinity |
| Description: |
20- and 25-hydroxy cholesterol, Cytosolic OSBP formed an approximately 440-kD oligomer with a member of the PTPPBS family of tyrosine phosphatases, Im et al, Moreira et al, Wang et al, a member of the OSBP-related protein (ORP) family, and 7-, and cholesterol, at 1, cholesterol, the serine/threonine phosphatase PP2A, (2001) refined the localization of the OSBP1 gene to chromosome 11q12, (2005) found that OSBP functions as a cholesterol-binding scaffolding protein coordinating the activity of 2 phosphatases to control the extracellular signal-regulated kinase (ERK) signaling pathway, (2005) reported the structure of the full-length yeast oxysterol-binding-protein Osh4, 1 by radiation hybrid analysis, 5- to 1, 9-angstrom resolution in complexes with ergosterol, Oxysterol-Binding Protein is a protein that in humans is encoded by the OSBP gene |
| Immunogen: |
mouse and rat), An amino acid sequence from the middle region of human Oxysterol-Binding Protein (WQKSLQYERDQRIRL) was used as the immunogen for this OSBP antibody (100% homologous in human |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the OSBP antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
OSBP |
| Short name: |
OSBP Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
OSBP (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
8503 |
| Gene: |
OSBP |
More about : OSBP |
| Long gene name: |
oxysterol binding protein |
| Synonyms: |
OSBP1 |
| Locus: |
11q12, 1 |
| Discovery year: |
1990-01-03 |
| GenBank acession: |
AF185696 |
| Entrez gene record: |
5007 |
| Classification: |
Pleckstrin homology domain containing Oxysterol binding proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000167422 |