| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
MFGE8 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Titration of the MFGE8 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This MFGE8 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q08431 |
| Purity: |
Antigen affinity |
| Description: |
Bu et al, By fluorescence in situ hybridization, Collins et al, Hanayama et al, It bound particularly strongly to cells expressing alpha-V-beta-3 integrin, It is a secreted protein found in vertebrates, MFGE8 specifically bound to apoptotic cells by recognizing aminophospholipids such as phosphatidylserine, Using a wound-healing assay, When engaged by phospholipids, also called Lactadherin or SED1, including mammals as well as birds, is a protein which in humans is encoded by the MFGE8 gene, it bound to cells via its RGD motif, they showed that the protein promoted migration of intestinal epithelial cells through a PKC-epsilon (PRKCE)-dependent mechanism, (1997) mapped the gene to chromosome 15q25, (2002) found that it is a factor that links apoptotic cells to phagocytes, (2007) showed that MFGE8 was expressed in intestinal lamina propria macrophages in mice, Milk Fat Globule-Egf Factor 8 |
| Immunogen: |
An amino acid sequence from the middle region of human MFGE8 (NGHEFDFIHDVNKKHK) was used as the immunogen for this MFGE8 antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the MFGE8 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
MFGE8 |
| Short name: |
MFGE8 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
milk fat globule-epidermal growth factor factor 8 protein (Antibody to) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
BA46 and EDIL1 and HMFG and hP47 and HsT19888 and MFG-E8 and MFGM and OAcGD3S and SED1 and SPAG10, Extracellular, MFGE8 and IDBG-28947 and ENSG00000140545 and 102723860, MFGE8 and IDBG-629001 and ENSBTAG00000003300 and 281913, Mfge8 and IDBG-193646 and ENSMUSG00000030605 and 17304, engulfment and biological process this GO :0007155 and cell adhesion and biological process this GO :0007338 and single fertilization and biological process this GO :0008429 and phosphatidylethanolamine binding and molecular function this GO :0009897 and external side of plasma membrane and cellular component this GO :0016020 and membrane and cellular component this GO :0016032 and viral process and biological process this GO :0019897 and extrinsic component of plasma membrane and cellular component this GO :0031012 and extracellular matrix and cellular component this GO :0050766 and positive regulation of pha this GO cytosis and biological process this GO :0070062 and extracellular vesicular exosome and cellular component this GO :2000427 and positive regulation of apoptotic cell clearance and biological process, phosphatidylethanolamine binding, recognition and biological process this GO :0006911 and pha this GO cytosis, this GO :0001525 and angiogenesis and biological process this GO :0001786 and phosphatidylserine binding and molecular function this GO :0005178 and integrin binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005615 and extracellular space and cellular component this GO :0006910 and pha this GO cytosis, this GO :0001786 : phosphatidylserine binding, this GO :0001786 : phosphatidylserine binding and also this GO :0005178 : integrin binding and also this GO :0005515 : protein binding and also this GO :0008429 : phosphatidylethanolamine binding, this GO :0005178 : integrin binding, this GO :0005515 : protein binding, this GO :0008429 : phosphatidylethanolamine binding, 4240, 102724554, milk fat globule-EGF factor 8 protein |
| Identity: |
7036 |
| Gene: |
MFGE8 |
More about : MFGE8 |
| Long gene name: |
milk fat globule-EGF factor 8 protein |
| Synonyms gene: |
SPAG10 |
| Synonyms gene name: |
sperm associated antigen 10 |
| Synonyms: |
SED1 EDIL1 BA46 OAcGD3S HsT19888 MFG-E8 hP47 |
| Synonyms name: |
sperm surface protein hP47 lactadherin |
| Locus: |
15q26, 1 |
| Discovery year: |
1996-10-31 |
| GenBank acession: |
U58516 |
| Entrez gene record: |
4240 |
| Pubmed identfication: |
9027496 19204935 |
| RefSeq identity: |
NM_005928 |
| Havana BLAST/BLAT: |
OTTHUMG00000148682 |