| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
CUL3 (Cullin-3) |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Notes: |
Titration of the CUL3 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This CUL3 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q13618 |
| Purity: |
Antigen affinity |
| Description: |
6 in humans, By immunoprecipitation analysis of HeLa cells and HCT116 human colon carcinoma cells expressing epitope-tagged proteins, Hartz (2012) mapped the gene to chromosome 2q36, KLHL12 interacted directly with CUL-3 and with the polymorphic intracellular loop-3 of D4, KLHL13, Kigoshi et al, Kipreos et al, Rondou et al, Sumara et al, and 3 in S, and CUL-3 interacted directly in a 370-kD protein complex in HeLa cell lysates, cerevisiae, designated cullins, with at least 5 members in nematodes, (1996) identified a conserved gene family, (2007) found that KLHL9, (2008) showed that interaction between KLHL12 and the CUL3 ubiquitin ligase complex directed ubiquitination of dopamine receptor D4(DRD4), (2011) showed that KLHL7 interacted with CUL-3 and ROC1(RBX1), 2 based on an alignment of the CUL3 sequence with the genomic sequence (GRCh37), Cullin-3 is a protein that in humans is encoded by the CUL3 gene |
| Immunogen: |
mouse and rat), An amino acid sequence from the middle region of human Cullin-3 (DVFERYYKQHLARRLLTNK) was used as the immunogen for this CUL3 antibody (100% homologous in human |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the CUL3 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
CUL3 (Cullin-3) |
| Short name: |
CUL3 Antibody (Cullin-3) |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
cullin 3 (Antibody to) (Cullin-3) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
BT, CUL-3 and PHA2E, CUL3 and IDBG-82331 and ENSG00000036257 and 8452, Cul3 and IDBG-173179 and ENSMUSG00000004364 and 26554, nuclei, protein heterodimerization activity, this GO :0000082 and G1/S transition of mitotic cell cycle and biological process this GO :0000090 and mitotic anaphase and biological process this GO :0000139 and this GO lgi membrane and cellular component this GO :0000209 and protein polyubiquitination and biological process this GO :0000902 and cell morphogenesis and biological process this GO :0000910 and cytokinesis and biological process this GO :0001701 and in utero embryonic development and biological process this GO :0001831 and trophectodermal cellular morphogenesis and biological process this GO :0004842 and ubiquitin-protein transferase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005794 and this GO lgi apparatus and cellular component this GO :0005827 and polar microtubule and cellular component this GO :0006511 and ubiquitin-dependent protein catabolic process and biological process this GO :0006513 and protein monoubiquitination and biological process this GO :0006888 and ER to this GO lgi vesicle-mediated transport and biological process this GO :0007050 and cell cycle arrest and biological process this GO :0007229 and integrin-mediated signaling pathway and biological process this GO :0007369 and gastrulation and biological process this GO :0008054 and cyclin catabolic process and biological process this GO :0008284 and positive regulation of cell proliferation and biological process this GO :0016020 and membrane and cellular component this GO :0016055 and Wnt signaling pathway and biological process this GO :0016477 and cell migration and biological process this GO :0016567 and protein ubiquitination and biological process this GO :0017145 and stem cell division and biological process this GO :0030332 and cyclin binding and molecular function this GO :0031208 and POZ domain binding and molecular function this GO :0031461 and cullin-RING ubiquitin ligase complex and cellular component this GO :0031463 and Cul3-RING ubiquitin ligase complex and cellular component this GO :0031625 and ubiquitin protein ligase binding and molecular function this GO :0032467 and positive regulation of cytokinesis and biological process this GO :0035024 and negative regulation of Rho protein signal transduction and biological process this GO :0040016 and embryonic cleavage and biological process this GO :0042803 and protein homodimerization activity and molecular function this GO :0043149 and stress fiber assembly and biological process this GO :0043161 and proteasome-mediated ubiquitin-dependent protein catabolic process and biological process this GO :0046982 and protein heterodimerization activity and molecular function this GO :0048208 and COPII vesicle coating and biological process this GO :0051322 and anaphase and biological process this GO :0070062 and extracellular vesicular exosome and cellular component this GO :0097193 and intrinsic apoptotic signaling pathway and biological process, this GO :0004842 : ubiquitin-protein transferase activity, this GO :0004842 : ubiquitin-protein transferase activity and also this GO :0005515 : protein binding and also this GO :0030332 : cyclin binding and also this GO :0031208 : POZ domain binding and also this GO :0031625 : ubiquitin protein ligase binding and also this GO :0042803 : protein homodimerization activity and also this GO :0046982 : protein heterodimerization activity, this GO :0005515 : protein binding, this GO :0030332 : cyclin binding, this GO :0031208 : POZ domain binding, this GO :0031625 : ubiquitin protein ligase binding, this GO :0042803 : protein homodimerization activity, this GO :0046982 : protein heterodimerization activity, 69487 and IDBG-644194 and ENSBTAG00000021769 and 534325, cullin 3 |
| Identity: |
2553 |
| Gene: |
CUL3 |
More about : CUL3 |
| Long gene name: |
cullin 3 |
| Locus: |
2q36, 2 |
| Discovery year: |
1998-10-29 |
| GenBank acession: |
U58089 |
| Entrez gene record: |
8452 |
| Pubmed identfication: |
8681378 17192413 |
| Classification: |
Cullins |
| Havana BLAST/BLAT: |
OTTHUMG00000133167 |