| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
SMARCB1 (BAF47) |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Titration of the SMARCB1 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This SMARCB1 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q12824 |
| Purity: |
Antigen affinity |
| Description: |
1998), By mutation analysis, It could bind independently with the protein phosphatase-1(PP1) catalytic subunit (PPP1CA) and stimulate its activity in solution and in complex with GADD34, SMARCB1 and PP1 did not compete for GADD34 binding, SNF5, The gene encodes a subunit of the SWI/SNF ATP-dependent chromatin-remodeling complex, The gene maps to chromosome 22q11, They suggested that it may function as a regulatory subunit of PP1, Wu et al, Wu et al, also known as BAF47, and INI1 , at least in part, but rather formed a stable trimeric complex with GADD34, either independently or together with GADD34, is a protein that in humans is encoded by the SMARCB1 gene, leading to inhibition of GADD34-mediated apoptosis, they showed that the GADD34 region homologous to the HSV-1 ICP34, through its interaction with SMARCB1, (2002) noted that GADD34 (PPP1R15A) and SNF5 can coexist in a trimeric complex with chimeric leukemic HRX (MLL) fusion proteins, (2002) proposed that GADD34 mediates growth suppression, 2(Versteege et al, 5 protein was necessary for interaction with SMARCB1/BAF47, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1) |
| Immunogen: |
mouse and rat), Amino acids 362-377 (EKKIRDQDRNTRRMRR) were used as the immunogen for this SMARCB1 antibody (100% homologous in human |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the SMARCB1 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
SMARCB1 (BAF47) |
| Short name: |
SMARCB1 Antibody (BAF47) |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
SMARCB1 (Antibody to) (BAF47) |
| Alternative technique: |
antibodies |
| Identity: |
11103 |
| Gene: |
SMARCB1 |
More about : SMARCB1 |
| Long gene name: |
SWI/SNF related, actin dependent regulator of chromatin, matrix associated, member 1 , subfamily b |
| Synonyms gene: |
SNF5L1 |
| Synonyms: |
BAF47 Ini1 Snr1 hSNFS Sfh1p RDT PPP1R144 |
| Synonyms name: |
homolog-like 1 integrase interactor 1 malignant rhabdoid tumor suppressor protein phosphatase 1, regulatory subunit 144 , sucrose nonfermenting, yeast |
| Locus: |
22q11, 23 |
| Discovery year: |
1995-08-21 |
| GenBank acession: |
U04847 |
| Entrez gene record: |
6598 |
| Pubmed identfication: |
7801128 10319872 10365963 |
| RefSeq identity: |
NM_003073 |
| Classification: |
Protein phosphatase 1 regulatory subunits |
| Havana BLAST/BLAT: |
OTTHUMG00000150737 |
| Locus Specific Databases: |
LRG_520 |