| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
NOX4 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Titration of the NOX4 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This NOX4 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q9JHI8 |
| Purity: |
Antigen affinity |
| Description: |
By FISH, By biochemical analysis of endogenous renal NOX4, Geiszt et al, Geiszt et al, In a note added in proof, Shiose et al, Shiose et al, They suggested that NOX4, also called Renal NADPH oxidase or RENOX, and a member of the NOX family of NADPH oxidases, as a renal source of ROS, is an enzyme that in humans is encoded by the NOX4 gene, may fulfill the function of the putative oxygen sensor in the kidney, they stated that genomic clones assigned to chromosome 11 also contain sequence corresponding to NADPH Oxidase 4, (2000) found that NIH 3T3 fibroblasts overexpressing the transfected protein showed increased production of superoxide and developed signs of cellular senescence, (2000) stated that the nucleotide sequence of NOX4 matches that found in a genomic clone on chromosome 15, (2001) determined that the enzyme can use either NADH or NADPH as an electron donor for superoxide production, (2001) mapped the gene to chromosome 11q14, 2-q21, NADPH oxidase 4 |
| Immunogen: |
An amino acid sequence from the C-terminus of mouse NADPH oxidase 4 (NRNNSYGTKFEYNKES) was used as the immunogen for this NOX4 antibody (100% rat homology) |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the NOX4 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
NOX4 |
| Short name: |
NOX4 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
NOX4 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
7891 |
| Gene: |
NOX4 |
More about : NOX4 |
| Long gene name: |
NADPH oxidase 4 |
| Synonyms: |
KOX-1 KOX |
| Locus: |
11q14, 3 |
| Discovery year: |
2000-05-30 |
| GenBank acession: |
AF254621 |
| Entrez gene record: |
50507 |
| RefSeq identity: |
NM_016931 |
| Havana BLAST/BLAT: |
OTTHUMG00000167298 |