| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
MAP3K1 (MEKK1) |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Titration of the MAP3K1 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This MAP3K1 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q13233 |
| Purity: |
Antigen affinity |
| Description: |
By assaying transfected COS-1 cells, MAP3K1 / MEKK1 directly and specifically interacted with JNKK1 (MAP2K4) and activated JNKK1 in cells and in vitro, MAPKKK1, MEK Kinase or MAP/ERK Kinase Kinase 1, MEKK1 and JNK1 competed for binding to JNKK1, Phosphorylation of JNKK1 disrupted their interaction, They mapped the human gene to chromosome 5 by somatic cell hybrid analysis, Using these allele-specific polymorphisms, Vinik et al, Xia et al, Xia et al, also known as MEKK1, but it had only a marginal effect on ERK2 (MAPK1), is an enzyme that in humans is encoded by the MAP3K1 gene, they mapped the mouse gene in an intersubspecific backcross to chromosome 13, (1995) identified DNA sequence and size polymorphisms in intronic and 3-prime untranslated regions of the mouse gene and the human homolog, (1998) concluded that JNKK1 is the preferred MEKK1 substrate, (1998) showed that human MAP3K1 / MEKK1 activated JNK1 (MAPK8) robustly and p38-alpha (MAPK14) less efficiently, Mitogen-activated protein kinase kinase kinase 1 |
| Immunogen: |
mouse and rat), An amino acid sequence from the C-terminus of human MEK kinase 1 (PEVLRGQQYGRSCDV) was used as the immunogen for this MAP3K1 antibody (100% homologous in human |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the MAP3K1 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
MAP3K1 (MEKK1) |
| Short name: |
MAP3K1 Antibody (MEKK1) |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
E3 ubiquitin protein ligase (Antibody to) (MEKK1), mitogen-activated protein phosphorylation catalyst kinase phosphorylation catalyst 1 |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
Cytoplasm, E3 ubiquitin protein ligase, MAP3K1 and IDBG-22000 and ENSG00000095015 and 4214, MAP3K1 and IDBG-629015 and ENSBTAG00000013790 and 523962, MAPKKK1 and MEKK and MEKK 1 and MEKK1 and SRXY6, Map3k1 and IDBG-182342 and ENSMUSG00000021754 and 26401, this GO :0000186 and activation of MAPKK activity and biological process this GO :0002224 and toll-like receptor signaling pathway and biological process this GO :0002755 and MyD88-dependent toll-like receptor signaling pathway and biological process this GO :0003382 and epithelial cell morphogenesis and biological process this GO :0004672 and protein kinase activity and molecular function this GO :0004709 and MAP kinase kinase kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0005488 and binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005829 and cytosol and cellular component this GO :0006468 and protein phosphorylation and biological process this GO :0007179 and transforming growth factor beta receptor signaling pathway and biological process this GO :0007254 and JNK cascade and biological process this GO :0007257 and activation of JUN kinase activity and biological process this GO :0008270 and zinc ion binding and molecular function this GO :0008545 and JUN kinase kinase activity and molecular function this GO :0008637 and apoptotic mitochondrial changes and biological process this GO :0016772 and transferase activity, this GO :0004672 : protein kinase activity, this GO :0004672 : protein kinase activity and also this GO :0004709 : MAP kinase kinase kinase activity and also this GO :0004713 : protein tyrosine kinase activity and also this GO :0005488 : binding and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0008270 : zinc ion binding and also this GO :0008545 : JUN kinase kinase activity and also this GO :0016772 : transferase activity, this GO :0004709 : MAP kinase kinase kinase activity, this GO :0004713 : protein tyrosine kinase activity, this GO :0005488 : binding, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0008270 : zinc ion binding, this GO :0008545 : JUN kinase kinase activity, this GO :0016772 : transferase activity, this GO :0019901 : protein kinase binding, transferase activity, transferring phosphorus-containing groups, transferring phosphorus-containing groups and also this GO :0019901 : protein kinase binding, transferring phosphorus-containing groups and molecular function this GO :0019901 and protein kinase binding and molecular function this GO :0030334 and regulation of cell migration and biological process this GO :0030838 and positive regulation of actin filament polymerization and biological process this GO :0034134 and toll-like receptor 2 signaling pathway and biological process this GO :0034142 and toll-like receptor 4 signaling pathway and biological process this GO :0034146 and toll-like receptor 5 signaling pathway and biological process this GO :0034162 and toll-like receptor 9 signaling pathway and biological process this GO :0034166 and toll-like receptor 10 signaling pathway and biological process this GO :0038095 and Fc-epsilon receptor signaling pathway and biological process this GO :0038123 and toll-like receptor TLR1:TLR2 signaling pathway and biological process this GO :0038124 and toll-like receptor TLR6:TLR2 signaling pathway and biological process this GO :0042060 and wound healing and biological process this GO :0043010 and camera-type eye development and biological process this GO :0045087 and innate immune response and biological process this GO :0061029 and eyelid development in camera-type eye and biological process this GO :0071260 and cellular response to mechanical stimulus and biological process, mitogen-activated protein kinase kinase kinase 1 |
| Identity: |
6848 |
| Gene: |
MAP3K1 |
More about : MAP3K1 |
| Long gene name: |
mitogen-activated protein kinase kinase kinase 1 |
| Synonyms gene: |
MEKK1 |
| Synonyms gene name: |
E3 ubiquitin protein ligase , mitogen-activated protein kinase kinase kinase 1 mitogen-activated protein kinase kinase kinase 1 |
| Synonyms: |
MEKK MAPKKK1 |
| Locus: |
5q11, 2 |
| Discovery year: |
1997-11-14 |
| GenBank acession: |
U29671 AF042838 |
| Entrez gene record: |
4214 |
| Pubmed identfication: |
8597633 |
| RefSeq identity: |
XM_042066 |
| Classification: |
Zinc fingers SWIM-type Mitogen-activated protein kinase kinase kinases |
| Havana BLAST/BLAT: |
OTTHUMG00000059486 |