| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
DCC |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Titration of the DCC antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This DCC antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P43146 |
| Purity: |
Antigen affinity |
| Description: |
Alterations in DCC occur frequently in colorectal cancer, DCC is a receptor or a component of a receptor that mediates the effects of netrin-1 on commissural axons, Heterozygous loss-of-function mutations in the gene can result in congenital mirror movements, It may function as a tumor-suppressor protein by inducing apoptosis in settings in which ligand is unavailable (for example, Stein et al, Studying a YAC contig containing the entire DCC coding region, The gene encodes a functional receptor for netrin and mediates axon outgrowth and the steering response, The protein can be detected in varying abundance in all specimens of normal colonic mucosa analyzed as well as in all specimens of adenomatous polyps, Vogelstein(1995) stated that the precise location of the gene was thought to be 18q21, and they complement genetic evidence for interactions between DCC and netrin homologs in C, colorectal carcinoma and colorectal liver metastases, during metastasis or tumor growth beyond local blood supply) through functional caspase cascades by a mechanism that requires cleavage of DCC at asp1290, they showed that the gene spans approximately 1, (2001) concluded that it plays a central role in netrin signaling of axon growth and guidance independent of A2B receptor activation, 3, 4 Mb, Deleted in Colorectal Carcinoma is also known as CRC18 or CRCR1 |
| Immunogen: |
Amino acids 162-178 (EVIGEPMPTIHWQKNQQ-human) were used as the immunogen for this DCC antibody (100% mouse homology) |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the DCC antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
DCC |
| Short name: |
DCC Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
deleted in colorectal carcinoma (Antibody to) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
CRC18 and CRCR1 and IGDCC1 and MRMV1 and NTN1R1, DCC and IDBG-3511 and ENSG00000187323 and 1630, Dcc and IDBG-157336 and ENSMUSG00000060534 and 13176, Plasma membranes, identical protein binding, this GO :0001764 and neuron migration and biological process this GO :0001975 and response to amphetamine and biological process this GO :0003713 and transcription coactivator activity and molecular function this GO :0004888 and transmembrane signaling receptor activity and molecular function this GO :0005042 and netrin receptor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006915 and apoptotic process and biological process this GO :0007409 and axonogenesis and biological process this GO :0007411 and axon guidance and biological process this GO :0010976 and positive regulation of neuron projection development and biological process this GO :0010977 and negative regulation of neuron projection development and biological process this GO :0016021 and integral component of membrane and cellular component this GO :0021965 and spinal cord ventral commissure morphogenesis and biological process this GO :0030424 and axon and cellular component this GO :0032584 and growth cone membrane and cellular component this GO :0033563 and dorsal/ventral axon guidance and biological process this GO :0033564 and anterior/posterior axon guidance and biological process this GO :0038007 and netrin-activated signaling pathway and biological process this GO :0042802 and identical protein binding and molecular function this GO :0042981 and regulation of apoptotic process and biological process this GO :0043065 and positive regulation of apoptotic process and biological process this GO :0045121 and membrane raft and cellular component this GO :0048671 and negative regulation of collateral sprouting and biological process this GO :0070374 and positive regulation of ERK1 and ERK2 cascade and biological process this GO :2000171 and negative regulation of dendrite development and biological process this GO :2001141 and regulation of RNA biosynthetic process and biological process, this GO :0003713 : transcription coactivator activity, this GO :0003713 : transcription coactivator activity and also this GO :0004888 : transmembrane signaling receptor activity and also this GO :0005042 : netrin receptor activity and also this GO :0005515 : protein binding and also this GO :0042802 : identical protein binding, this GO :0004888 : transmembrane signaling receptor activity, this GO :0005042 : netrin receptor activity, this GO :0005515 : protein binding, this GO :0042802 : identical protein binding, deleted in colorectal carcinoma |
| Identity: |
2701 |
| Gene: |
DCC |
More about : DCC |
| Long gene name: |
DCC netrin 1 receptor |
| Synonyms gene name: |
deleted in colorectal carcinoma |
| Synonyms: |
IGDCC1 NTN1R1 |
| Synonyms name: |
DCC subclass, immunoglobulin superfamily, member 1 |
| Locus: |
18q21, 2 |
| Discovery year: |
1990-05-25 |
| GenBank acession: |
X76132 |
| Entrez gene record: |
1630 |
| Pubmed identfication: |
2294591 24400119 |
| RefSeq identity: |
NM_005215 |
| Classification: |
I-set domain containing Immunoglobulin like domain containing Fibronectin type III domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000132698 |
| Locus Specific Databases: |
LRG_1107 |