| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
CYP1A2 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Titration of the CYP1A2 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This CYP1A2 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P05177 |
| Purity: |
Antigen affinity |
| Description: |
CYP1A2 accounts for nearly 15% of the cytochrome P450 in the human liver, CYP1A2 is a member of the cytochrome P450 superfamily of enzymes, Cigarette smoking has also been shown to increase its activity, Expression of the protein appears to be induced by various dietary constituents, Ikeya et al, In humans, Inducers of CYP1A2 include cruciferous vegetables, It displays higher activity in men than in women, It localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons(PAHs), Polypeptide 2 is a member of the cytochrome P450 mixed-function oxidase system and is involved in the metabolism of xenobiotics in the body, Subfamily I, The gene encodes a P450 enzyme involved in O-deethylation of phenacetin, The gene is mapped on 15q24, and is inhibited by oral contraceptives, some of which are found in cigarette smoke, the enzyme is encoded by the CYP1A2 gene, (1989) found that the human gene spans almost 7, 1, 8 kb and contains 7 exons, Cytochrome P450 |
| Immunogen: |
An amino acid sequence from the middle region of human Cytochrome P450 1A2 (QKTVQEHYQDFDKNSVRD) was used as the immunogen for this CYP1A2 antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the CYP1A2 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
CYP1A2 |
| Short name: |
CYP1A2 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
CYP1A2 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
2596 |
| Gene: |
CYP1A2 |
More about : CYP1A2 |
| Long gene name: |
cytochrome P450 family 1 subfamily A member 2 |
| Synonyms gene name: |
cytochrome P450, family 1, polypeptide 2 , polypeptide 2 cytochrome P450, subfamily A, subfamily I (aromatic compound-inducible) |
| Synonyms: |
P3-450 CP12 |
| Locus: |
15q24, 1 |
| Discovery year: |
1990-04-25 |
| GenBank acession: |
AF182274 |
| Entrez gene record: |
1544 |
| Pubmed identfication: |
15128046 |
| RefSeq identity: |
NM_000761 |
| Classification: |
Cytochrome P450 family 1 |
| Havana BLAST/BLAT: |
OTTHUMG00000172901 |
| Locus Specific Databases: |
Human Cytochrome P450 (CYP) Allele Nomenclature Committee |