| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
GATA2 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Notes: |
Titration of the GATA2 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This GATA2 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P23769 |
| Purity: |
Antigen affinity |
| Description: |
By exon trapping using a PAC contig spanning a breakpoint region associated with myeloid leukemia, Ciciotte et al, GATA2 is expressed in hematopoietic progenitors, GATA2 is transcribed from telomere to centromere, They pointed out that the human gene had been mapped to chromosome 3 by Dorfman et al, Wieser et al, and also in nonhematopoietic embryonic stem cells, and megakaryocytes, have emerged as candidate regulators of gene expression in hematopoietic cells, including early erythroid cells, mast cells, which contain zinc fingers in their DNA binding domain, (1992) using DNA from a panel of 12 rodent/human hybrids containing various human chromosomes and applying Southern blot analysis, (1997) mapped the mouse GATA2 gene to chromosome 6 by study of DNA from an interspecific backcross panel, (2000) mapped the GATA2 gene to chromosome 3q21, The GATA family of transcription factors |
| Immunogen: |
mouse and rat), An amino acid sequence from the N-terminus of human GATA2 (DVFFNHLDSQGNPYY) was used as the immunogen for this GATA2 antibody (100% homologous in human |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the GATA2 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
GATA2 |
| Short name: |
GATA2 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
GATA binding protein 2 (Antibody to) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
C2H2 zinc finger domain binding, DCML and IMD21 and MONOMAC and NFE1B, DNA-templated and biological process this GO :0006355 and regulation of transcription, DNA-templated and biological process this GO :0006909 and pha this GO cytosis and biological process this GO :0007596 and blood coagulation and biological process this GO :0008134 and transcription factor binding and molecular function this GO :0008270 and zinc ion binding and molecular function this GO :0021514 and ventral spinal cord interneuron differentiation and biological process this GO :0021533 and cell differentiation in hindbrain and biological process this GO :0021902 and commitment of neuronal cell to specific neuron type in forebrain and biological process this GO :0021954 and central nervous system neuron development and biological process this GO :0021983 and pituitary gland development and biological process this GO :0030154 and cell differentiation and biological process this GO :0030182 and neuron differentiation and biological process this GO :0035019 and somatic stem cell maintenance and biological process this GO :0035065 and regulation of histone acetylation and biological process this GO :0035854 and eosinophil fate commitment and biological process this GO :0042472 and inner ear morphogenesis and biological process this GO :0043234 and protein complex and cellular component this GO :0043565 and sequence-specific DNA binding and molecular function this GO :0045599 and negative regulation of fat cell differentiation and biological process this GO :0045638 and negative regulation of myeloid cell differentiation and biological process this GO :0045648 and positive regulation of erythrocyte differentiation and biological process this GO :0045650 and negative regulation of macrophage differentiation and biological process this GO :0045654 and positive regulation of megakaryocyte differentiation and biological process this GO :0045746 and negative regulation of Notch signaling pathway and biological process this GO :0045766 and positive regulation of angiogenesis and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0048469 and cell maturation and biological process this GO :0048663 and neuron fate commitment and biological process this GO :0048873 and homeostasis of number of cells within a tissue and biological process this GO :0050766 and positive regulation of pha this GO cytosis and biological process this GO :0060216 and definitive hemopoiesis and biological process this GO :0060872 and semicircular canal development and biological process this GO :0070345 and negative regulation of fat cell proliferation and biological process this GO :0070742 and C2H2 zinc finger domain binding and molecular function this GO :0097154 and GABAergic neuron differentiation and biological process this GO :2000178 and negative regulation of neural precursor cell proliferation and biological process this GO :2000977 and regulation of forebrain neuron differentiation and biological process, GATA2 and IDBG-55213 and ENSG00000179348 and 2624, GATA2 and IDBG-646502 and ENSBTAG00000019707 and 506809, Gata2 and IDBG-166776 and ENSMUSG00000015053 and 14461, nuclei, this GO :0000122 and negative regulation of transcription from RNA polymerase II promoter and biological process this GO :0000980 and RNA polymerase II distal enhancer sequence-specific DNA binding and molecular function this GO :0001077 and RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription and molecular function this GO :0001158 and enhancer sequence-specific DNA binding and molecular function this GO :0001655 and urogenital system development and biological process this GO :0001709 and cell fate determination and biological process this GO :0001892 and embryonic placenta development and biological process this GO :0003677 and DNA binding and molecular function this GO :0003682 and chromatin binding and molecular function this GO :0003700 and sequence-specific DNA binding transcription factor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0006351 and transcription, this GO :0000980 : RNA polymerase II distal enhancer sequence-specific DNA binding, this GO :0000980 : RNA polymerase II distal enhancer sequence-specific DNA binding and also this GO :0001077 : RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription and also this GO :0001158 : enhancer sequence-specific DNA binding and also this GO :0003677 : DNA binding and also this GO :0003682 : chromatin binding and also this GO :0003700 : sequence-specific DNA binding transcription factor activity and also this GO :0005515 : protein binding and also this GO :0008134 : transcription factor binding and also this GO :0008270 : zinc ion binding and also this GO :0043565 : sequence-specific DNA binding and also this GO :0070742 : C2H2 zinc finger domain binding, this GO :0001077 : RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription, this GO :0001158 : enhancer sequence-specific DNA binding, this GO :0003677 : DNA binding, this GO :0003682 : chromatin binding, this GO :0003700 : sequence-specific DNA binding transcription factor activity, this GO :0005515 : protein binding, this GO :0008134 : transcription factor binding, this GO :0008270 : zinc ion binding, this GO :0043565 : sequence-specific DNA binding, this GO :0070742 : C2H2 zinc finger domain binding, GATA binding protein 2 |
| Identity: |
4171 |
| Gene: |
GATA2 |
More about : GATA2 |
| Long gene name: |
GATA binding protein 2 |
| Synonyms gene name: |
GATA-binding protein 2 |
| Synonyms: |
NFE1B |
| Locus: |
3q21, 3 |
| Discovery year: |
1992-11-03 |
| GenBank acession: |
AF169253 |
| Entrez gene record: |
2624 |
| Pubmed identfication: |
1714909 |
| RefSeq identity: |
NM_032638 |
| Classification: |
GATA zinc finger domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000159689 |
| Locus Specific Databases: |
LRG_295 |