| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
CUL2 Cullin-2 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Notes: |
Titration of the CUL2 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This Cullin 2 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q13617 |
| Purity: |
Antigen affinity |
| Description: |
By fluorescence in situ hybridization, CUL2 is one of several proteins required for degradation of a class of RNA-binding germline proteins in somatic cells of the early blastomere, Clifford et al, Lonergan et al, Pause et al, Using immunofluorescence, a region reported to show loss of heterozygosity (LOH) in several forms of human cancer, as has been proposed for CUL1 (603134), including non-clear cell renal cell carcinoma, it was shown that CUL2 is a cytosolic protein that can be translocated to the nucleus by VHL, (1997) suggested that CUL2 is a candidate tumor suppressor gene, (1998) demonstrated that formation of the VBC-CUL2 complexes is linked to the regulation of hypoxia-inducible mRNAs by VHL, (1999) mapped the gene to 10p11, 1, 2-p11, Cullin-2 is a protein that in humans is encoded by the CUL2 gene |
| Immunogen: |
An amino acid sequence from the middle region of human Cullin-2 (HECQQRMVADHLQFLHA) was used as the immunogen for this CUL2 antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the CUL2 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
CUL2 Cullin-2 |
| Short name: |
CUL2 Antibody Cullin-2 |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
cullin 2 (Antibody to) Cullin-2 |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
CUL2 and IDBG-637219 and ENSBTAG00000007133 and 535219, CUL2 and IDBG-69264 and ENSG00000108094 and 8453, Cul2 and IDBG-127755 and ENSMUSG00000024231 and 71745, nuclei, protein complex binding, this GO :0000082 and G1/S transition of mitotic cell cycle and biological process this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0006511 and ubiquitin-dependent protein catabolic process and biological process this GO :0007050 and cell cycle arrest and biological process this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0016032 and viral process and biological process this GO :0016567 and protein ubiquitination and biological process this GO :0030163 and protein catabolic process and biological process this GO :0030891 and VCB complex and cellular component this GO :0031461 and cullin-RING ubiquitin ligase complex and cellular component this GO :0031462 and Cul2-RING ubiquitin ligase complex and cellular component this GO :0031625 and ubiquitin protein ligase binding and molecular function this GO :0032403 and protein complex binding and molecular function this GO :0061418 and regulation of transcription from RNA polymerase II promoter in response to hypoxia and biological process this GO :0071456 and cellular response to hypoxia and biological process this GO :0097193 and intrinsic apoptotic signaling pathway and biological process, this GO :0005515 : protein binding, this GO :0005515 : protein binding and also this GO :0031625 : ubiquitin protein ligase binding and also this GO :0032403 : protein complex binding, this GO :0031625 : ubiquitin protein ligase binding, this GO :0032403 : protein complex binding, cullin 2 |
| Identity: |
2552 |
| Gene: |
CUL2 |
More about : CUL2 |
| Long gene name: |
cullin 2 |
| Locus: |
10p11, 21 |
| Discovery year: |
1998-10-29 |
| GenBank acession: |
U83410 |
| Entrez gene record: |
8453 |
| Pubmed identfication: |
8681378 |
| RefSeq identity: |
NM_003591 |
| Classification: |
Cullins |
| Havana BLAST/BLAT: |
OTTHUMG00000017950 |