| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Nrf2 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ICC, IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Immunocytochemistry: 0, Western blot: 0 |
| Notes: |
Titration of the Nrf2 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting points |
| Intented use: |
This Nrf2 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q16236 |
| Purity: |
Antigen affinity |
| Description: |
NFE2, NFE2L1, NFE2L2 induces the expression of various genes including those that encode for several antioxidant enzymes, Oncogene-directed increased expression of the protein is a mechanism for the activation of the Nrf2 antioxidant program evident in primary cells and tissues of mice expressing KRas (G12D) and BRaf (V619E), The identification of somatic mutations that disrupt the NFE2L2-KEAP1 interaction to stabilize NFE2L2 and increase the constitutive transcription of target genes indicated that enhanced reactive oxygen species (ROS) detoxification and additional NFE2L2 functions may in fact be tumorigenic, and NFE2L2 comprise a family of human genes encoding basic leucine zipper (bZIP) transcription factors, and in human pancreatic cancer, and it may play a physiological role in the regulation ofoxidative stress, is a transcription factor that in humans is encoded by the NFE2L2 gene, NFE2L2 (nuclear factor erythroid-derived 2-like 2) also known as NRF2 or NFE2-related transcription factor 2 |
| Immunogen: |
mouse and rat), Amino acids 580-596 (EYSLQQTRDGNVFLVPK) were used as the immunogen for this Nrf2 antibody (100% homologous in human |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the Nrf2 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Nrf2 |
| Short name: |
Nrf2 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
Nrf2 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
7782 |
| Gene: |
NFE2L2 |
More about : NFE2L2 |
| Long gene name: |
erythroid 2 like 2 , nuclear factor |
| Synonyms gene name: |
erythroid 2-like 2 , nuclear factor (erythroid-derived 2)-like 2 nuclear factor |
| Synonyms: |
NRF2 |
| Synonyms name: |
NF-E2-related factor 2 |
| Locus: |
2q31, 2 |
| Discovery year: |
1994-03-24 |
| Entrez gene record: |
4780 |
| Pubmed identfication: |
7937919 |
| RefSeq identity: |
NM_006164 |
| Classification: |
Basic leucine zipper proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000133620 |