| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
CrkL |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Notes: |
Titration of the CrkL antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This CrkL antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P46109 |
| Purity: |
Antigen affinity |
| Description: |
By fluorescence in situ hybridization, CrkLwas required for normal cellular responses to Fgf8, Erk activation, Senechal et al, The authors also found CrkL to be a substrate for the BCR-ABL tyrosine kinase, While CrkL is not the human homolog of CRK, activates Ras-dependent and JNK pathways, also called CrkL, and target gene expression, and transforms fibroblasts, centromeric of the chronic myelogenous leukemia breakpoint region, encodes a 303-amino acid polypeptide with a predicted molecular mass of ~36 kD, including survival and migration, it is similar to protein-tyrosine kinases with SH2 and SH3 (src homology) domains, leading them to conclude that it is a tyrosine kinase and an oncogene, (1996)showed that CRKL becomes phosphorylated when overexpressed, the gene is localized to chromosome 22q11, v-Crk sarcoma virus CT10 oncogene homolog(avian)-like) |
| Immunogen: |
Amino acids 286-303 (FPFTHVKIFDPQNPDENE-human) were used as the immunogen for this CrkL antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the CrkL antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
CrkL |
| Short name: |
CrkL Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
v-crk sarcoma virus CT10 oncogene homolog (avian)-like (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
2363 |
| Gene: |
CRKL |
More about : CRKL |
| Long gene name: |
CRK like proto-oncogene, adaptor protein |
| Synonyms gene name: |
v-crk avian sarcoma virus CT10 oncogene homolog-like |
| Locus: |
22q11, 21 |
| Discovery year: |
1994-09-14 |
| Entrez gene record: |
1399 |
| Pubmed identfication: |
8361759 8798523 |
| RefSeq identity: |
NM_005207 |
| Classification: |
SH2 domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000150807 |