| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
HEXA |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Titration of the HEXA antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This HEXA antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P06865 |
| Purity: |
Antigen affinity |
| Description: |
Arg-424, Chimeric constructs were expressed in HeLa cells and selected constructs were produced in the baculovirus expression system to determine their ability to degrade GM2 ganglioside in the presence of GM2 activator protein, Even though the alpha and beta subunits of hexosaminidase A can both cleave GalNAc residues, Hexosaminidase A and the cofactor GM2 activator protein catalyze the degradation of the GM2 gangliosides and other molecules containing terminal N-acetyl hexosamines The HEXA gene encodes the alpha subunit of hexosaminidase A, The alpha subunit contains a key residue, Their results allowed them to define two noncontiguous sequences in the alpha subunit (amino acids 1-191 and 403-529) which, a lysosomal enzyme involved in the breakdown of gangliosides, conferred activity against the sulfated substrate, only the alpha subunit is able to hydrolyze GM2 gangliosides, when substituted into analogous positions in the beta subunit, which is essential for binding the N-acetyl-neuramanic residue of GM2 gangliosides, HEXA (Hexosaminidase A) is an enzyme that in humans is encoded by the HEXA gene |
| Immunogen: |
alpha subunit (DVMAYNKLNVFHWHLVD) was used as the immunogen for this HEXA antibody, An amino acid sequence from the middle region of human Hexosaminidase A |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the HEXA antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
HEXA |
| Short name: |
HEXA Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
HEXA (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
4878 |
| Gene: |
HEXA |
More about : HEXA |
| Long gene name: |
hexosaminidase subunit alpha |
| Synonyms gene name: |
hexosaminidase A (alpha polypeptide) |
| Synonyms name: |
Tay Sachs disease GM2 gangliosidosis beta-hexosaminidase subunit alpha |
| Locus: |
15q23 |
| Discovery year: |
2001-06-22 |
| GenBank acession: |
M13520 |
| Entrez gene record: |
3073 |
| Pubmed identfication: |
2952641 3013851 |
| RefSeq identity: |
NM_000520 |
| Havana BLAST/BLAT: |
OTTHUMG00000133445 |
| Locus Specific Databases: |
Hexosaminidase A, Tay-Sachs Disease ALSOD, the Amyotrophic Lateral Sclerosis Online Genetic Database |