| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
DCI |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ICC, IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Immunocytochemistry: 0, Western blot: 0 |
| Notes: |
Titration of the DCI antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This DCI antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P42126 |
| Purity: |
Antigen affinity |
| Description: |
All classes of enoyl-CoA isomerases belong to a family of enzymes, Dodecenoyl-CoA Delta Isomerase/DCI is involved in the beta-oxidation, It does so by shifting the position of the double bonds in the acyl-CoA intermediates and converting 3-cis or trans-enoyl-CoA to 2-trans-enoyl-CoA, It plays a particularly important role in the metabolism of unsaturated fatty acids, also known as 3, and when examined with x-ray crystallography, each turn consisting of two beta-sheets and one alpha-helix, exhibit a common structural feature of the family, is an enzyme that catalyzes the conversion of cis-or trans-double bonds of fatty acids at gamma-carbon(position 3) to trans double bonds at beta-carbon(position 2), of unsaturated fatty acids with double bonds at odd-numbered carbon positions, one of the most frequently used pathways in fatty acid degradation, the N-terminal core with a spiral fold composed of four turns, the hydratase/isomerase or crotonase superfamily, 2-trans-enoyl-CoA isomerase (ECI1), Dodecenoyl-CoA Delta Isomerase |
| Immunogen: |
An amino acid sequence from the C-terminus of human DCI (ADVQNFVSFISKDSIQKSL) was used as the immunogen for this DCI antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the DCI antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
DCI |
| Short name: |
DCI Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
DCI (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
2703 |
| Gene: |
ECI1 |
More about : ECI1 |
| Long gene name: |
enoyl-CoA delta isomerase 1 |
| Synonyms gene: |
DCI |
| Synonyms gene name: |
dodecenoyl-Coenzyme A delta isomerase (3, 2 trans-enoyl-Coenzyme A isomerase) dodecenoyl-CoA isomerase |
| Synonyms name: |
3, 2 trans-enoyl-CoA isomerase |
| Locus: |
16p13, 3 |
| Discovery year: |
1994-02-23 |
| Entrez gene record: |
1632 |
| Pubmed identfication: |
7829074 |
| Havana BLAST/BLAT: |
OTTHUMG00000128830 |