| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
ERp57 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Titration of the ERp57 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This ERp57 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P30101 |
| Purity: |
Antigen affinity |
| Description: |
ERp57 or ER60, Hirano et al, It is also part of the major histocompatibility complex(MHC) class I peptide-loading complex, Koivunen et al, The PDIA3 protein has protein disulfide isomerase activity, They showed that the expression level increased after oncogenic transformation of normal rat kidney cells and NIH 3T3 cells, When coexpressed with alpha prolyl 4-hydroxylase, also called GRP58, expressed human ERp57 and found that the protein had a thiol-dependent reductase activity, is an isomerase enzyme, it did not form prolyl 4-hydroxylase tetramers, mapped on 15q15, member 3), nor did it have prolyl 4-hydroxylase activity, showed that PDIA3/ERp57 could not substitute for the beta subunit of PDI, which is essential for formation of the final antigen conformation and export from the endoplasmic reticulum to the cell surface, 3, PDIA3 (Protein disulfide isomerase family A |
| Immunogen: |
mouse and rat), Amino acids 172-188 (LKAASNLRDNYRFAHTN) were used as the immunogen for this ERp57 antibody (100% homologous in human |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the ERp57 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
ERp57 |
| Short name: |
ERp57 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
ERp57 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
4606 |
| Gene: |
PDIA3 |
More about : PDIA3 |
| Long gene name: |
protein disulfide isomerase family A member 3 |
| Synonyms gene: |
GRP58 |
| Synonyms gene name: |
58kDa protein disulfide isomerase-associated 3 protein disulfide isomerase family A, glucose regulated protein, member 3 |
| Synonyms: |
P58 ERp61 ERp57 ERp60 GRP57 PI-PLC HsT17083 |
| Locus: |
15q15, 3 |
| Discovery year: |
1997-06-09 |
| Entrez gene record: |
2923 |
| Pubmed identfication: |
8974399 |
| RefSeq identity: |
NM_005313 |
| Classification: |
Protein disulfide isomerases |
| Havana BLAST/BLAT: |
OTTHUMG00000044444 |