| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Collagen IV (COL4A1) |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Notes: |
Titration of the Collagen IV antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This Collagen IV antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P02462 |
| Purity: |
Antigen affinity |
| Description: |
Like the other members of the type IV collagen gene family, Mutations in COL4A1 exons 24 and 25 are associated with HANAC(autosomal dominant hereditary angiopathy with nephropathy, Type IV collagen is the major structural component of glomerular basement membranes(GBM), and muscle cramps), forming a'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter, Collagen alpha-1(IV) chain is a protein that in humans is encoded by the COL4A1 gene, aneurysms |
| Immunogen: |
An amino acid sequence from the N-terminus of human Collagen IV COL4A1 (QAQVQEKGDFATKGEKGQK) was used as the immunogen for this Collagen IV antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the Collagen IV antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Collagen IV (COL4A1) |
| Short name: |
Collagen IV Antibody (COL4A1) |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
a 1), classification IV, Collagen IV (Antibody to) (collagen |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
COL4A1 and IDBG-50258 and ENSG00000187498 and 1282, COL4A1 and IDBG-633705 and ENSBTAG00000012849 and 282191, Col4a1 and IDBG-132200 and ENSMUSG00000031502 and 12826, Extracellular, alpha 1, arresten and HANAC and ICH and POREN1, platelet-derived growth factor binding, this GO :0001569 and patterning of blood vessels and biological process this GO :0005201 and extracellular matrix structural constituent and molecular function this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005581 and collagen trimer and cellular component this GO :0005587 and collagen type IV trimer and cellular component this GO :0005604 and basement membrane and cellular component this GO :0005788 and endoplasmic reticulum lumen and cellular component this GO :0007411 and axon guidance and biological process this GO :0007420 and brain development and biological process this GO :0007528 and neuromuscular junction development and biological process this GO :0022617 and extracellular matrix disassembly and biological process this GO :0030023 and extracellular matrix constituent conferring elasticity and molecular function this GO :0030198 and extracellular matrix organization and biological process this GO :0030574 and collagen catabolic process and biological process this GO :0030855 and epithelial cell differentiation and biological process this GO :0031012 and extracellular matrix and cellular component this GO :0048407 and platelet-derived growth factor binding and molecular function this GO :0048514 and blood vessel morphogenesis and biological process this GO :0061304 and retinal blood vessel morphogenesis and biological process this GO :0061333 and renal tubule morphogenesis and biological process this GO :0071230 and cellular response to amino acid stimulus and biological process this GO :0071711 and basement membrane organization and biological process, this GO :0005201 : extracellular matrix structural constituent, this GO :0005201 : extracellular matrix structural constituent and also this GO :0005515 : protein binding and also this GO :0030023 : extracellular matrix constituent conferring elasticity and also this GO :0048407 : platelet-derived growth factor binding, this GO :0005515 : protein binding, this GO :0030023 : extracellular matrix constituent conferring elasticity, this GO :0048407 : platelet-derived growth factor binding, type IV, collagen |
| Identity: |
2202 |
| Gene: |
COL4A1 |
More about : COL4A1 |
| Long gene name: |
collagen type IV alpha 1 chain |
| Synonyms gene name: |
alpha 1 , collagen, type IV |
| Locus: |
13q34 |
| Discovery year: |
2001-06-22 |
| GenBank acession: |
J04217 |
| Entrez gene record: |
1282 |
| Pubmed identfication: |
3691802 |
| Classification: |
Collagens |
| Havana BLAST/BLAT: |
OTTHUMG00000017342 |
| Locus Specific Databases: |
LRG_1116 |