| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Caspase-1 (p10) |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Titration of the Caspase-1 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting points |
| Intented use: |
This Caspase-1 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P29466 |
| Purity: |
Antigen affinity |
| Description: |
Caspase-1 has been shown to induce cell necrosis or pyroptosis and may function in various developmental stages, IL-18, It belongs to a family of cysteine proteases known as caspases that always cleave proteins following an aspartic acid residue, It was purified from the cytosol of the THP monocytic cell line and found that the active protease was made up of two peptides, The CASP1 gene is mapped to 11q23, a site frequently involved in rearrangement in human cancers, an apoptosis-related cysteine protease, and IL-33 precursor proteins, by Southern DNA blot analysis of rodent-human hybrids and by in situ hybridization to normal metaphase chromosomes, including a number of leukemias and lymphomas, is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta, which they called p20 and p10 based on their apparent molecular masses by SDS-PAGE, Caspase-1 |
| Immunogen: |
Amino acids 249-262 (VFIGRLIEHMQEYA) from the large subunit were used as the immunogen for this Caspase-1 antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the Caspase-1 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Caspase-1 (p10) |
| Short name: |
Caspase-1 Antibody (p10) |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
caspase-1 (Antibody to) (p10) |
| Alternative technique: |
antibodies |