| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
BSP Bone Sialoprotein |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Titration of the BSP antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This BSP antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P21815 |
| Purity: |
Antigen affinity |
| Description: |
Bone sialoprotein is a predicted 35KD acidic glycoprotein that undergoes extensive posttranslational modifications and is visualized by western blot at up to 70KD, It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, The BSP gene is mapped to 4q22, The only extraskeletal site of its synthesis is the trophoblast, This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, also known as Bone sialoprotein and BSP, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor, and osteoclasts, including hypertrophic chondrocytes, is a major structural protein of the bone matrix, osteoblasts, osteocytes, 1, Integrin-binding sialoprotein |
| Immunogen: |
An amino acid sequence from the N-terminus of human Bone Sialoprotein (FSMKNLHRRVKIEDSEEN) was used as the immunogen for this BSP antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the BSP antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
BSP Bone Sialoprotein |
| Short name: |
BSP Antibody Bone Sialoprotein |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
BSP (Antibody to) Bone Sialoprotein |
| Alternative technique: |
antibodies |
| Identity: |
5341 |
| Gene: |
IBSP |
More about : IBSP |
| Long gene name: |
integrin binding sialoprotein |
| Synonyms: |
BSP SP-II BSP-II |
| Synonyms name: |
bone sialoprotein bone sialoprotein II |
| Locus: |
4q22, 1 |
| Discovery year: |
1993-03-23 |
| Entrez gene record: |
3381 |
| Pubmed identfication: |
8406493 |
| Classification: |
SIBLING family |
| Havana BLAST/BLAT: |
OTTHUMG00000130600 |